Document Detail


Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells.
MedLine Citation:
PMID:  20459219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We present the light scattering of individual Plasmodium falciparum-parasitized human red blood cells (Pf-RBCs), and demonstrate progressive alterations to the scattering signal arising from the development of malaria-inducing parasites. By selectively imaging the electric fields using quantitative phase microscopy and a Fourier transform light scattering technique, we calculate the light scattering maps of individual Pf-RBCs. We show that the onset and progression of pathological states of the Pf-RBCs can be clearly identified by the static scattering maps. Progressive changes to the biophysical properties of the Pf-RBC membrane are captured from dynamic light scattering.
Authors:
YongKeun Park; Monica Diez-Silva; Dan Fu; Gabriel Popescu; Wonshik Choi; Ishan Barman; Subra Suresh; Michael S Feld
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  15     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-05-12     Completed Date:  2010-09-13     Revised Date:  2011-07-28    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  020506     Citation Subset:  IM    
Affiliation:
Massachusetts Institute of Technology, G. R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. ykpark@mit.edu
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MeSH Terms
Descriptor/Qualifier:
Erythrocytes / parasitology*,  pathology*
Humans
Light
Nephelometry and Turbidimetry / methods*
Plasmodium falciparum / pathogenicity,  physiology*
Reference Values
Scattering, Radiation
Grant Support
ID/Acronym/Agency:
1R01HL094270-01A1/HL/NHLBI NIH HHS; P41-RR02594-18/RR/NCRR NIH HHS
Comments/Corrections

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