Document Detail


State of differentiation defines buccal epithelial cell affinity for cross-linking to Candida albicans Hwp1.
MedLine Citation:
PMID:  17686003     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Candida albicans utilizes mammalian cell-associated transglutaminase (TGase) activity to adhere covalently to human buccal epithelial cells (BECs) through Hyphal Wall Protein 1. Little is known about the factors leading to the identity and appearance of Hwp1 binding partners on cells lining the oral cavity. The observation that BECs vary in their ability to attach to C. albicans germ tubes and to bind recombinant Hwp1 (rHwp1) suggested that differentiation may play a role in affinity for germ tube attachment. Individual BECs were characterized for differentiation status and rHwp1 binding. rHwp1 bound to the more terminally differentiated cells displaying SPR3 and keratin 13 but not to less differentiated cells with abundant involucrin. Sequential expression of involucrin followed by SPR3 in oral keratinocytes was demonstrated using stratified organotypic cultures and a feeder layer system with the OKF6/TERT-2 cell line. Increased cross-linking of the lysine analogue 5-(biotinamido)pentylamine to cultured OKF6/TERT-2 cell proteins accompanied this increased expression of SPR3. Western blot analysis demonstrated the presence of rHwp1 cross-links to proteins from BECs or from OKF6/TERT-2 cells that had been mechanically dislodged from culture dishes. Therefore, the differentiation of SPR3 positive from involucrin positive cells is correlated with the acquisition of affinity for cross-linking to rHwp1 and covalent adhesion of germ tubes to BECs.
Authors:
Gomathinayagam Ponniah; Christiane Rollenhagen; Yong-Sun Bahn; Janet F Staab; Paula Sundstrom
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  36     ISSN:  0904-2512     ISO Abbreviation:  J. Oral Pathol. Med.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-09     Completed Date:  2007-12-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  456-67     Citation Subset:  D; IM    
Affiliation:
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA.
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MeSH Terms
Descriptor/Qualifier:
Amines / metabolism
Biotin / analogs & derivatives,  metabolism
Candida albicans / physiology*
Cell Adhesion / physiology
Cell Cycle Proteins / metabolism
Cell Differentiation
Cell Line
Cells, Cultured
Epithelial Cells / cytology,  metabolism
Fungal Proteins / analysis,  physiology*
Humans
Hyphae / physiology
Keratin-13 / metabolism
Keratinocytes / metabolism
Membrane Glycoproteins / analysis,  physiology*
Mouth Mucosa / cytology*,  metabolism
Protein Binding
Protein Precursors / metabolism
Transglutaminases / physiology
Grant Support
ID/Acronym/Agency:
DE 011375/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Amines; 0/Cell Cycle Proteins; 0/Fungal Proteins; 0/HWP1 protein, Candida albicans; 0/Keratin-13; 0/Membrane Glycoproteins; 0/Protein Precursors; 115416-38-1/5-(biotinamido)pentylamine; 58-85-5/Biotin; 60108-77-2/involucrin; EC 2.3.2.13/Transglutaminases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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