Document Detail


Starved Tetrahymena thermophila cells that are unable to mount an effective heat shock response selectively degrade their rRNA.
MedLine Citation:
PMID:  6504043     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tetrahymena thermophila cells that had been shifted from log growth to a non-nutrient medium (60 mM Tris) were unable, during the first few hours of starvation, to mount a successful heat shock response and were killed by what should normally have been a nonlethal heat shock. An examination of the protein synthetic response of these short-starved cells during heat shock revealed that whereas they were able to initiate the synthesis of heat shock proteins, it was at a much reduced rate relative to controls and they quickly lost all capacity to synthesize any proteins. Certain pretreatments of cells, including a prior heat shock, abolished the heat shock inviability of these starved cells. Also, if cells were transferred to 10 mM Tris rather than 60 mM Tris, they were not killed by the same heat treatment. We found no abnormalities in either heat shock or non-heat shock mRNA metabolism in starved cells unable to survive a sublethal heat shock when compared with the response of those cells which can survive such a treatment. However, selective rRNA degradation occurred in the nonsurviving cells during the heat shock and this presumably accounted for their inviability. A prior heat shock administered to growing cells not only immunized them against the lethality of a heat shock while starved, but also prevented rRNA degradation from occurring.
Authors:
R L Hallberg; K W Kraus; R C Findly
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  4     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1985-01-10     Completed Date:  1985-01-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2170-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Electrophoresis, Polyacrylamide Gel
Heat-Shock Proteins / biosynthesis*,  genetics
RNA, Messenger / metabolism
RNA, Ribosomal / metabolism*
Tetrahymena / genetics*,  growth & development
Chemical
Reg. No./Substance:
0/Heat-Shock Proteins; 0/RNA, Messenger; 0/RNA, Ribosomal
Comments/Corrections

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