Document Detail

Starvation and temperature upshift cause an increase in the enzymatically active cell wall-associated glyceraldehyde-3-phosphate dehydrogenase protein in yeast.
MedLine Citation:
PMID:  14654434     Owner:  NLM     Status:  MEDLINE    
The cell wall-associated glyceraldehyde-3-phosphate dehydrogenase (cwGAPDH) activity in Saccharomyces cerevisiae increases (two- to 10-fold, depending on the strain) in response to starvation and temperature upshift. Assays using transformants carrying pTDH, a yeast centromer derivative plasmid containing the Candida albicans TDH3 gene (encoding GAPDH) fused in frame with the yeast SUC2-coding region for internal invertase, showed that starvation and/or temperature upshift result in a similar increase in both cwGAPDH and cell wall-associated invertase activities. In addition, this incorporation of GAPDH protein into the cell wall in response to stress does not require (i) de novo protein synthesis, indicating that preexisting cytosolic enzyme is incorporated into the cell wall, (ii) nor the participation of the ubiquitin yeast stress response system, as no differences were observed between wild-type and polyubiquitin-depleted (Deltaubi4) strains.
María Luisa Delgado; María Luisa Gil; Daniel Gozalbo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS yeast research     Volume:  4     ISSN:  1567-1356     ISO Abbreviation:  FEMS Yeast Res.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-05     Completed Date:  2004-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101085384     Medline TA:  FEMS Yeast Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  297-303     Citation Subset:  IM    
Departament de Microbiologia i Ecologia, Universitat de València, Avgda. Vicent Andrés Estellés s/n, 46100, Burjassot, Spain.
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MeSH Terms
Candida albicans / enzymology
Cell Wall / enzymology*
Fungal Proteins / analysis
Gene Expression Regulation, Fungal
Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism*
Saccharomyces cerevisiae / enzymology
Starvation / enzymology*
Reg. No./Substance:
0/Fungal Proteins; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases

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