Document Detail

Starvation-induced cleavage of the tRNA anticodon loop in Tetrahymena thermophila.
MedLine Citation:
PMID:  16272149     Owner:  NLM     Status:  MEDLINE    
Amino acid deprivation triggers dramatic physiological responses in all organisms, altering both the synthesis and destruction of RNA and protein. Here we describe, using the ciliate Tetrahymena thermophila, a previously unidentified response to amino acid deprivation in which mature transfer RNA (tRNA) is cleaved in the anticodon loop. We observed that anticodon loop cleavage affects a small fraction of most or all tRNA sequences. Accumulation of cleaved tRNA is temporally coordinated with the morphological and metabolic changes of adaptation to starvation. The starvation-induced endonucleolytic cleavage activity targets tRNAs that have undergone maturation by 5' and 3' end processing and base modification. Curiously, the majority of cleaved tRNAs lack the 3' terminal CCA nucleotides required for aminoacylation. Starvation-induced tRNA cleavage is inhibited in the presence of essential amino acids, independent of the persistence of other starvation-induced responses. Our findings suggest that anticodon loop cleavage may reduce the accumulation of uncharged tRNAs as part of a specific response induced by amino acid starvation.
Suzanne R Lee; Kathleen Collins
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-11-03
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  280     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-26     Completed Date:  2006-02-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  42744-9     Citation Subset:  IM    
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA.
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MeSH Terms
Amino Acids / chemistry
Amino Acyl-tRNA Synthetases / chemistry
Anticodon / chemistry*
Blotting, Northern
Dose-Response Relationship, Drug
Nucleic Acid Conformation*
RNA / chemistry
RNA, Complementary / chemistry
RNA, Transfer / chemistry*
Reverse Transcriptase Polymerase Chain Reaction
Tetrahymena thermophila / metabolism*
Time Factors
Reg. No./Substance:
0/Amino Acids; 0/Anticodon; 0/RNA, Complementary; 63231-63-0/RNA; 9014-25-9/RNA, Transfer; EC 6.1.1.-/Amino Acyl-tRNA Synthetases

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