Document Detail


Starvation-induced changes in the cell surface of Azospirillum lipoferum.
MedLine Citation:
PMID:  10922497     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Three starvation regimes (a deficient culture medium, a saline buffer solution and distilled water) were evaluated for their possible effect on cell surface characteristics of Azospirillum lipoferum 1842 related to the initial adsorption of the bacterium to surfaces. The bacteria survived for 7 days in all media although they did not multiply. Upon transfer from a rich growth medium (nutrient agar) to starvation conditions, cell surface hydrophobicity dropped sharply but recovered its initial value within 24 to 48 h, except in phosphate-buffered saline, the length of the recovery period depending on the starvation medium. Starvation affected the sugar affinity of the A. lipoferum cell surface mainly towards p-aminophenyl-alpha-D-mannopyranoside, to a lesser extent to glucose, but not to other monosaccharides tested. Starvation changed the concentration of several cell surface proteins but did not induce the synthesis of new ones. The cell surface hydrophobic protein (43 kDa) of A. lipoferum 1842 was unaffected by any starvation treatment for a period of up to 48 h, but later disappeared. These data showed that starvation is not a major factor in inducing changes in the cell surface which lead to the primary phase of attachment of Azospirillum to surfaces.
Authors:
Thelma Castellanos; Felipe Ascencio; Yoav Bashan
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  FEMS microbiology ecology     Volume:  33     ISSN:  1574-6941     ISO Abbreviation:  FEMS Microbiol. Ecol.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2004-Jun-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8901229     Medline TA:  FEMS Microbiol Ecol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-9     Citation Subset:  -    
Affiliation:
Environmental Microbiology, The Center for Biological Research of the Northwest (CIB), A.P. 128 23000 La Paz, Baja California Sur, Mexico.
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