Document Detail


Starch granule initiation and growth are altered in barley mutants that lack isoamylase activity.
MedLine Citation:
PMID:  12100486     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two mutant lines of barley, Risø 17 and Notch-2, were found to accumulate phytoglycogen in the grain. Like the sugary mutants of maize and rice, these phytoglycogen-accumulating mutants of barley lack isoamylase activity in the developing endosperm. The mutants were shown to be allelic, and to have lesions in the isoamylase gene, isa1 that account for the absence of this enzyme. As well as causing a reduction in endosperm starch content, the mutations have a profound effect on the structure, number and timing of initiation of starch granules. There are no normal A-type or B-type granules in the mutants. The mutants have a greater number of starch granules per plastid than the wild-type and, particularly in Risø 17, this leads to the appearance of compound starch granules. These results suggest that, as well as suppressing phytoglycogen synthesis, isoamylase in the wild-type endosperm plays a role in determining the number, and hence the form, of starch granules.
Authors:
Rachel A Burton; Helen Jenner; Luke Carrangis; Brendan Fahy; Geoffrey B Fincher; Chris Hylton; David A Laurie; Mary Parker; Darren Waite; Sonja van Wegen; Tamara Verhoeven; Kay Denyer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  31     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-07-08     Completed Date:  2002-09-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  97-112     Citation Subset:  IM    
Affiliation:
Department of Plant Science, University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF490375;  AF490376;  AF490377
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MeSH Terms
Descriptor/Qualifier:
Alleles
Amino Acid Sequence
Base Sequence
Chromosome Mapping
Cloning, Molecular
DNA, Complementary / genetics
DNA, Plant / genetics
Genes, Plant
Glucans / metabolism
Hordeum / genetics*,  growth & development,  metabolism*,  ultrastructure
Isoamylase / genetics*
Microscopy, Electron
Molecular Sequence Data
Mutation
Plastids / metabolism
Starch / metabolism*
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Plant; 0/Glucans; 9005-25-8/Starch; EC 3.2.1.68/Isoamylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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