Document Detail


Staphylococcus rostri sp. nov., a haemolytic bacterium isolated from the noses of healthy pigs.
MedLine Citation:
PMID:  19819995     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Twenty coagulase-negative Staphylococcus strains displaying alpha-haemolysis (delta-haemolysin) on sheep-blood agar were isolated from the noses of different pigs in Switzerland. The strains were Gram-stain-positive, non-motile cocci, catalase-positive and coagulase-negative. Sequence analysis of the 16S rRNA gene, sodA, rpoB, dnaJ and hsp60 and phylogenetic characteristics revealed that the strains showed the closest relatedness to Staphylococcus microti CCM 4903(T) and Staphylococcus muscae DSM 7068(T). The strains can be differentiated from S. microti by the absence of mannose fermentation and arginine arylamidase and from S. muscae by the absence of beta-glucuronidase activity and production of alkaline phosphatase. The chosen type strain ARI 262(T) shared 20.1 and 31.9 % DNA relatedness with S. microti DSM 22147(T) and S. muscae CCM 4903(T), respectively, by DNA-DNA hybridization. iso-C(15 : 0), anteiso-C(15 : 0) and iso-C(17 : 0) were the most common fatty acids. Cell-wall structure analysis revealed the peptidoglycan type A3alpha l-Lys-Gly(2)-l-Ser-Gly (type A11.3). The presence of teichoic acid was determined by sequencing the N-acetyl-beta-d-mannosaminyltransferase gene tarA, which is involved in biosynthesis of ribitol teichoic acid. Menaquinone 7 (MK-7) was the predominant respiratory quinone. The G+C content of ARI 262(T) was 38.8 mol%. The isolated strains represent a novel species of the genus Staphylococcus, for which we propose the name Staphylococcus rostri sp. nov. The type strain is ARI 262(T) (=DSM 21968(T) =CCUG 57266(T)) and strain ARI 602 (=DSM 21969 =CCUG 57267) is a reference strain.
Authors:
Andrea Riesen; Vincent Perreten
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Publication Detail:
Type:  Journal Article     Date:  2009-10-09
Journal Detail:
Title:  International journal of systematic and evolutionary microbiology     Volume:  60     ISSN:  1466-5026     ISO Abbreviation:  Int. J. Syst. Evol. Microbiol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-08     Completed Date:  2010-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100899600     Medline TA:  Int J Syst Evol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2042-7     Citation Subset:  IM    
Affiliation:
Institute of Veterinary Bacteriology, University of Berne, Berne, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / metabolism
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Fatty Acids / metabolism
Hemolysin Proteins / metabolism
Hemolysis
Molecular Sequence Data
Nose / microbiology*
Phylogeny
RNA, Ribosomal, 16S / genetics
Staphylococcus / classification*,  genetics,  isolation & purification*,  metabolism
Sus scrofa / microbiology*
Switzerland
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/DNA, Ribosomal; 0/Fatty Acids; 0/Hemolysin Proteins; 0/RNA, Ribosomal, 16S; 74838-20-3/delta hemolysin protein, Staphylococcus aureus

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