Document Detail

Staphylococcus epidermidis polysaccharide intercellular adhesin activates complement.
MedLine Citation:
PMID:  22077230     Owner:  NLM     Status:  In-Data-Review    
Staphylococcus epidermidis is a frequent cause of nosocomial infections. The central virulence factor of S. epidermidis is biofilm formation. Polysaccharide intercellular adhesin (PIA) constitutes the major biofilm matrix-component. PIA and biofilm have been implicated in S. epidermidis evasion of host immune defence. We examined the effects of S. epidermidis PIA on the inflammatory response with focus on complement activation. We used a human whole-blood ex vivo model of infection and compared the effects of a PIA-positive S. epidermidis strain (SE1457) and its PIA-negative isogenic mutant (M10). The independent effect of purified PIA on complement activation was investigated. In glucose-rich media, the mutant formed a proteinacious DNA-rich biofilm, whereas SE1457 formed a thick PIA-biofilm. In biofilm growth, SE1457 induced a stronger activation of the complement system compared with M10. We verified that purified PIA was independently responsible for a strong activation of the complement system. In contrast, M10 induced higher granulocyte activation by expression of CD11b and higher secretion of cytokines. We conclude that PIA has potent pro-inflammatory properties by activating the complement system. However, in a complex balance of the immune response, the decreased activation of granulocytes and cytokines by a PIA biofilm may limit host eradication of S. epidermidis.
Elizabeth G Aarag Fredheim; Hildegunn Norbakken Granslo; Trond Flægstad; Yngve Figenschau; Holger Rohde; Irina Sadovskaya; Tom Eirik Mollnes; Claus Klingenberg
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEMS immunology and medical microbiology     Volume:  63     ISSN:  1574-695X     ISO Abbreviation:  FEMS Immunol. Med. Microbiol.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315554     Medline TA:  FEMS Immunol Med Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  269-80     Citation Subset:  IM    
Copyright Information:
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Paediatric Research Group, Faculty of Health Sciences, University of Tromsø, Tromsø, Norway.
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