Document Detail


Staphylococcus cohnii hemolysins - isolation, purification and properties.
MedLine Citation:
PMID:  19381478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A total 355 of Staphylococcus cohnii isolates from hospital environment, patients (newborns), medical staff and from non-hospital environment were tested for hemolytic activity. Ninety-one % of S. cohnii ssp. cohnii and 74.5 % S. cohnii ssp. urealyticus strains exhibited hemolysis synergistic to S. aureus ATCC 25923 strain. Crude preparations of hemolysins of both bacterial subspecies presented delta-hemolysin, but not alpha- and beta-toxin activity. Highly pure hemolysins were obtained by semipreparative SDS-PAGE or by organic solvent extraction from the freeze-dried crude preparations. Native-PAGE and 2D-PAGE showed their high heterogeneity. Molar masses of single hemolysin units estimated by the Tris-Tricine-SDS-PAGE were calculated as 3.47 kDa for S. cohnii ssp. cohnii and 3.53 kDa for S. cohnii ssp. urealyticus.
Authors:
M Rózalska; E M Szewczyk
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-18
Journal Detail:
Title:  Folia microbiologica     Volume:  53     ISSN:  1874-9356     ISO Abbreviation:  Folia Microbiol. (Praha)     Publication Date:  2008  
Date Detail:
Created Date:  2009-04-21     Completed Date:  2009-05-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376757     Medline TA:  Folia Microbiol (Praha)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  521-6     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Microbiology, Medical University of Łódź, Poland. mrozalska@pharm.am.lodz.pl
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MeSH Terms
Descriptor/Qualifier:
Adult
Cross Infection / microbiology
Electrophoresis, Polyacrylamide Gel
Hemolysin Proteins / isolation & purification*,  metabolism
Humans
Infant, Newborn
Isoelectric Focusing
Molecular Weight
Personnel, Hospital
Poland
Skin / microbiology
Staphylococcal Infections / microbiology
Staphylococcus / chemistry*,  isolation & purification
Chemical
Reg. No./Substance:
0/Hemolysin Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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