Document Detail


Staphylococcus aureus and Bacillus subtilis W23 make polyribitol wall teichoic acids using different enzymatic pathways.
MedLine Citation:
PMID:  21035733     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Wall teichoic acids (WTAs) are anionic polymers that play key roles in bacterial cell shape, cell division, envelope integrity, biofilm formation, and pathogenesis. B. subtilis W23 and S. aureus both make polyribitol-phosphate (RboP) WTAs and contain similar sets of biosynthetic genes. We use in vitro reconstitution combined with genetics to show that the pathways for WTA biosynthesis in B. subtilis W23 and S. aureus are different. S. aureus requires a glycerol-phosphate primase called TarF in order to make RboP-WTAs; B. subtilis W23 contains a TarF homolog, but this enzyme makes glycerol-phosphate polymers and is not involved in RboP-WTA synthesis. Instead, B. subtilis TarK functions in place of TarF to prime the WTA intermediate for chain extension by TarL. This work highlights the enzymatic diversity of the poorly characterized family of phosphotransferases involved in WTA biosynthesis in Gram-positive organisms.
Authors:
Stephanie Brown; Timothy Meredith; Jonathan Swoboda; Suzanne Walker
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Chemistry & biology     Volume:  17     ISSN:  1879-1301     ISO Abbreviation:  Chem. Biol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-11-01     Completed Date:  2011-02-22     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  9500160     Medline TA:  Chem Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1101-10     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Bacillus subtilis / enzymology*
Bacterial Proteins / metabolism*
Phosphotransferases / metabolism
Polysaccharides / chemistry,  metabolism
Staphylococcus aureus / enzymology*
Teichoic Acids / biosynthesis*
Grant Support
ID/Acronym/Agency:
1P01AI083214/AI/NIAID NIH HHS; 5R01M078477//PHS HHS; F3178727//PHS HHS; P01 AI083214/AI/NIAID NIH HHS; R01 GM078477/GM/NIGMS NIH HHS; R01 GM078477-04/GM/NIGMS NIH HHS; T32 AI007061/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Polysaccharides; 0/Teichoic Acids; 51584-96-4/polyribitol phosphate; EC 2.7.-/Phosphotransferases
Comments/Corrections

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