| Staphylococcus aureus and Bacillus subtilis W23 make polyribitol wall teichoic acids using different enzymatic pathways. | |
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MedLine Citation:
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PMID: 21035733 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Wall teichoic acids (WTAs) are anionic polymers that play key roles in bacterial cell shape, cell division, envelope integrity, biofilm formation, and pathogenesis. B. subtilis W23 and S. aureus both make polyribitol-phosphate (RboP) WTAs and contain similar sets of biosynthetic genes. We use in vitro reconstitution combined with genetics to show that the pathways for WTA biosynthesis in B. subtilis W23 and S. aureus are different. S. aureus requires a glycerol-phosphate primase called TarF in order to make RboP-WTAs; B. subtilis W23 contains a TarF homolog, but this enzyme makes glycerol-phosphate polymers and is not involved in RboP-WTA synthesis. Instead, B. subtilis TarK functions in place of TarF to prime the WTA intermediate for chain extension by TarL. This work highlights the enzymatic diversity of the poorly characterized family of phosphotransferases involved in WTA biosynthesis in Gram-positive organisms. |
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Authors:
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Stephanie Brown; Timothy Meredith; Jonathan Swoboda; Suzanne Walker |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Chemistry & biology Volume: 17 ISSN: 1879-1301 ISO Abbreviation: Chem. Biol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-11-01 Completed Date: 2011-02-22 Revised Date: 2012-10-09 |
Medline Journal Info:
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Nlm Unique ID: 9500160 Medline TA: Chem Biol Country: United States |
Other Details:
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Languages: eng Pagination: 1101-10 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
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enzymology* Bacterial Proteins / metabolism* Phosphotransferases / metabolism Polysaccharides / chemistry, metabolism Staphylococcus aureus / enzymology* Teichoic Acids / biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
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1P01AI083214/AI/NIAID NIH HHS; 5R01M078477//PHS HHS; F3178727//PHS HHS; P01 AI083214-016668/AI/NIAID NIH HHS; R01 GM078477-04/GM/NIGMS NIH HHS; T32 AI007061/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Polysaccharides; 0/Teichoic Acids; 51584-96-4/polyribitol phosphate; EC 2.7.-/Phosphotransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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