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Staphylococcal superantigen-like protein 10 (SSL10) binds to phosphatidylserine and apoptotic cells.
MedLine Citation:
PMID:  22486378     Owner:  NLM     Status:  Publisher    
Staphylococcal superantigen-like proteins (SSLs) are a family of exoproteins which share structural similarity with staphylococcal superantigens. Although SSLs do not have superantigenic activity, part of them were reported to bind to host immune related molecules and implied to be involved in the immune evasion of S aureus. In this study, we showed that SSL10 is capable of binding to phospholipids. SSL10 bound to phosphatidylserine (PS) containing liposome, but not to phosphatidylcholine (PC) liposome. SSL10 but not SSL7 bound to PS containing liposome, suggesting that SSL10 specifically binds to PS. Analyzing PS binding ability among recombinant truncated SSL10 fragments revealed that β-barrel in the N-terminal oligonucleotide/oligosaccharide-binding (OB)-fold domain contributed to the PS binding capacity. FITC labeled OB-fold of SSL10 stained hydrogen peroxide treated Jurkat cells. Annexin V is widely utilized for detection of apoptosis. The OB-fold domain of SSL10 also bound to apoptotic cells in the presence of EDTA, unlike annexin V, suggesting that the OB-fold of SSL10 recognizes PS and apoptotic cells in a Ca(2+) independent manner. These findings suggest SSL10 and its derived peptides as a novel detection tool for apoptotic cells.
Saotomo Itoh; Ryosuke Yokoyama; Chizuko Murase; Takemasa Takii; Tsutomu Tsuji; Kikuo Onozaki
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-4
Journal Detail:
Title:  Microbiology and immunology     Volume:  -     ISSN:  1348-0421     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Societies and Blackwell Publishing Asia Pty Ltd.
Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1, Tanabe-Dori, Mizuho-ku, Nagoya, 460-8603, Japan, Department of Microbiology, Graduate School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
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