Document Detail

Standards for total serum protein assays--a collaborative study.
MedLine Citation:
PMID:  1169135     Owner:  NLM     Status:  MEDLINE    
We have studied the standardization of total serum protein assay with the biuret reaction. Standard solutions were prepared from lyophilized preparations of human serum albumin and bovine serum albumin, with corrections made for volatile material and ash contents. These solutions and a solution of crystalline albumin standard were analyzed with a new stable biuret reagent, to establish absorptivity values (values for the absorbance of a 1 g/liter final reaction mixture). The mean values obtained were 0.302, 0.292, and 0.290 for human serum albumin, bovine serum albumin, and the crystalline albumin, respectively. We believe that the established absorptivity value will improve the accuracy of serum protein determinations. We studied the linearity of the relation between color produced and protein concentration, with use of the solutions described above and a serum pool. The color adheres to Beer's law up to the highest concentration tested: 3 g/liter for HSA and BSA, and 2.8 g/liter for serum in the final reaction mixture. The new biuret reagent has been stable for one year at room temperature. We recommend the use of bovine serum albumin as a primary standard for serum protein assays. It is inexpensive, easily available, and exhibits the best linearity in the biuret reaction.
B T Doumas
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  21     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1975 Jul 
Date Detail:
Created Date:  1975-09-09     Completed Date:  1975-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1159-66     Citation Subset:  IM    
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MeSH Terms
Biuret Reaction / standards
Blood Proteins / analysis*
Carbon Dioxide / pharmacology
Glucose / pharmacology
Serum Albumin / standards
Serum Albumin, Bovine / standards
Reg. No./Substance:
0/Blood Proteins; 0/Serum Albumin; 0/Serum Albumin, Bovine; 124-38-9/Carbon Dioxide; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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