Document Detail

Standardized method for high-resolution 1H-NMR of cerebrospinal fluid.
MedLine Citation:
PMID:  7729054     Owner:  NLM     Status:  MEDLINE    
This study describes a standardized method for recording single-pulse 1H-nuclear magnetic resonance (1H-NMR) spectra from cerebrospinal fluid (CSF). Quantitative data for alanine, valine, threonine, and lactic acid correlated well with data obtained with conventional techniques. The pH of the samples is important for the reproducibility of the chemical shift of resonances, and should be standardized to improve recognition and assignment of resonances. A database of resonances from various metabolites is presented. Fifty compounds could be identified in CSF, 15 of which had not been observed earlier in NMR studies of CSF. We describe for the first time in the literature, to our knowledge, 3-hydroxyisovaleric acid as a regular component of many CSF samples. As examples of the diagnostic power of the technique, spectra are shown of CSF from patients with three different inborn errors of metabolism. We found high concentrations of N-acetylaspartic acid, citric acid, and succinic acid in CSF from a patient with Canavan disease. This is indirect evidence for the existence of a carrier mechanism that is shared by these di- and tricarboxylic acids.
R A Wevers; U Engelke; U Wendel; J G de Jong; F J Gabreëls; A Heerschap
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical chemistry     Volume:  41     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-05-30     Completed Date:  1995-05-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  744-51     Citation Subset:  IM    
Institute of Neurology, University Hospital Nijmegen, The Netherlands.
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MeSH Terms
Amino Acids / cerebrospinal fluid
Canavan Disease / cerebrospinal fluid
Carbon-Carbon Ligases*
Cerebrospinal Fluid / chemistry*
Gas Chromatography-Mass Spectrometry
Histidine / blood
Hydrogen-Ion Concentration
Lactates / cerebrospinal fluid
Lactic Acid
Ligases / deficiency
Magnetic Resonance Spectroscopy / methods*
Metabolism, Inborn Errors / cerebrospinal fluid
Sensitivity and Specificity
Valerates / cerebrospinal fluid
Reg. No./Substance:
0/Amino Acids; 0/Lactates; 0/Valerates; 50-21-5/Lactic Acid; 625-08-1/beta-hydroxyisovaleric acid; 71-00-1/Histidine; EC 6.-/Ligases; EC 6.4.-/Carbon-Carbon Ligases; EC carboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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