Document Detail


Standardized Seidel test to evaluate different sutureless cataract incision configurations.
MedLine Citation:
PMID:  20494775     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To compare aqueous outflow and India ink inflow to evaluate clear corneal incisions (CCIs). SETTING: Wilmer Eye Institute, Baltimore, Maryland, USA. METHODS: Two 25-gauge needles connected to a salt solution bag and to a digital manometer were inserted through the limbus of human donor corneas 120 degrees apart from each other. Three 2.75 mm wide incisions were created in a different third of each cornea as follows: single-plane 1.50 mm tunnel length, single-plane 3.00 mm tunnel length, and 2-step 3.00 mm tunnel length. The Seidel test was evaluated at 5 intraocular pressures (IOPs) in the physiologic range. A masked observer evaluated the recorded tests. With a preset 10 mm Hg IOP, India ink was applied to the incision site and a sudden IOP fluctuation induced. India ink influx was outlined and measured by planimetry. RESULTS: With the 1.5 mm single-plain incisions, all 6 globes showed inflow and outflow. With the 3.0 mm single-plain incisions, all 6 globes showed inflow and 2 showed outflow by the Seidel test. With the 3.0 mm 2-step incisions, 2 globes showed inflow and 3 had positive Seidel test results. Area and length of inflow were statistically significantly greater with the 2 single-planed incisions than with the 2-step incisions. CONCLUSION: This human ex vivo model showed that wound deformation produced during Seidel testing might not be an accurate way to predict the risk for bacterial invasion in the early postoperative period.
Authors:
William N May; Juan Castro-Combs; Gilherme G Quinto; Renata Kashiwabuchi; Emily W Gower; Ashley Behrens
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cataract and refractive surgery     Volume:  36     ISSN:  1873-4502     ISO Abbreviation:  J Cataract Refract Surg     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-07-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8604171     Medline TA:  J Cataract Refract Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1011-7     Citation Subset:  IM    
Copyright Information:
(c) 2010 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.
Affiliation:
Doheny Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, California, USA. williamnmaymd@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Aqueous Humor / secretion*
Carbon / metabolism*
Cataract Extraction / methods
Cornea / metabolism,  surgery*
Diagnostic Techniques, Ophthalmological / standards*
Eye Infections, Bacterial / diagnosis
Fluorescein / diagnostic use
Humans
Intraocular Pressure
Models, Biological*
Surgical Wound Dehiscence / diagnosis*,  metabolism
Tissue Donors
Video Recording
Wound Healing / physiology
Chemical
Reg. No./Substance:
0/chinese ink; 2321-07-5/Fluorescein; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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