| Standardization and quality controls for the methylated DNA immunoprecipitation technique. | |
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MedLine Citation:
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PMID: 22507898 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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MeDIP (Methylated DNA Immunoprecipitation) is a relatively recent technique aimed to enrich the methylated fraction of DNA with an antibody directed against 5-methyl-cytosine. MeDIP processed samples are suitable for investigation of the methylation status of specific genomic loci and for performing genome-wide screening when hybridized to DNA methylation microarrays or analyzed by deep sequencing. Here, we describe a standardization protocol and quality controls to assess the specificity, reproducibility and efficiency of the MeDIP procedure. These may have utility when comparing results between samples and experiments within laboratories and between laboratories. |
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Authors:
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Sofia Lisanti; Thomas von Zglinicki; John Mathers |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-01 |
Journal Detail:
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Title: Epigenetics : official journal of the DNA Methylation Society Volume: 7 ISSN: 1559-2308 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-4-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101265293 Medline TA: Epigenetics Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Centre for Integrated Systems Biology of Aging and Nutrition; Institute for Aging and Health; Newcastle University; Newcastle upon Tyne, UK; Human Nutrition Research Centre; Institute for Aging & Health; Newcastle University; Newcastle upon Tyne, UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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