Document Detail


Standardization of a colorimetric method to quantify growth and metabolic activity of Wolbachia-infected mosquito cells.
MedLine Citation:
PMID:  18622662     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Aedes albopictus Aa23 cell line, which is persistently infected with Wolbachia pipientis strain wAlbB, tends to grow as aggregated clusters of cells that are difficult to disperse for conventional quantification based on cell number. We used A. albopictus C7-10 cells to validate conversion of methylthiazole tetrazolium (MTT) to a colored formazan product with respect to incubation time, cell number over a 40-fold range, and metabolic activity as cells enter stationary phase. Using this assay, we showed that the doubling time of Aa23 cells increases from about 45 h early after plating to more than 70 h as the cells reach stationary levels. Growth of Aa23 cells proceeds at similar rates in the presence or absence of tetracycline concentrations that decrease the abundance of Wolbachia. Insofar as the MTT assay reflects mitochondrial function, our results indicate that, in Aa23 cells, abundance of intracellular Wolbachia has no measurable effect on mitochondrial activity in the presence of tetracycline.
Authors:
Ann M Fallon; Vanessa J Hellestad
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-07-12
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  44     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:    2008 Sep-Oct
Date Detail:
Created Date:  2008-10-29     Completed Date:  2009-01-29     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  United States    
Other Details:
Languages:  eng     Pagination:  351-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Colorimetry / methods,  standards*
Culicidae / metabolism*,  microbiology*
Mitochondria / metabolism
Reference Standards
Tetracycline / pharmacology
Time Factors
Wolbachia* / drug effects
Grant Support
ID/Acronym/Agency:
AI 070913/AI/NIAID NIH HHS; R21 AI070913/AI/NIAID NIH HHS; R21 AI070913-02/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
F8VB5M810T/Tetracycline
Comments/Corrections

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