| Standardising analysis of carbon monoxide rebreathing for application in anti-doping. | |
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MedLine Citation:
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PMID: 20832358 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Determination of total haemoglobin mass (Hb(mass)) via carbon monoxide (CO) depends critically on repeatable measurement of percent carboxyhaemoglobin (%HbCO) in blood with a hemoximeter. The main aim of this study was to determine, for an OSM3 hemoximeter, the number of replicate measures as well as the theoretical change in percent carboxyhaemoglobin required to yield a random error of analysis (Analyser Error) of ≤1%. Before and after inhalation of CO, nine participants provided a total of 576 blood samples that were each analysed five times for percent carboxyhaemoglobin on one of three OSM3 hemoximeters; with approximately one-third of blood samples analysed on each OSM3. The Analyser Error was calculated for the first two (duplicate), first three (triplicate) and first four (quadruplicate) measures on each OSM3, as well as for all five measures (quintuplicates). Two methods of CO-rebreathing, a 2-min and 10-min procedure, were evaluated for Analyser Error. For duplicate analyses of blood, the Analyser Error for the 2-min method was 3.7, 4.0 and 5.0% for the three OSM3s when the percent carboxyhaemoglobin increased by two above resting values. With quintuplicate analyses of blood, the corresponding errors reduced to .8, .9 and 1.0% for the 2-min method when the percent carboxyhaemoglobin increased by 5.5 above resting values. In summary, to minimise the Analyser Error to ∼≤1% on an OSM3 hemoximeter, researchers should make ≥5 replicates of percent carboxyhaemoglobin and the volume of CO administered should be sufficient increase percent carboxyhaemoglobin by ≥5.5 above baseline levels. |
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Authors:
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Anthony C Alexander; Laura A Garvican; Caroline M Burge; Sally A Clark; James S Plowman; Christopher J Gore |
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Publication Detail:
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Type: Journal Article Date: 2010-09-15 |
Journal Detail:
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Title: Journal of science and medicine in sport / Sports Medicine Australia Volume: 14 ISSN: 1878-1861 ISO Abbreviation: J Sci Med Sport Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9812598 Medline TA: J Sci Med Sport Country: Australia |
Other Details:
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Languages: eng Pagination: 100-5 Citation Subset: IM |
Copyright Information:
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Crown Copyright © 2010. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Physiology, Australian Institute of Sport, Australia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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