Document Detail

Staining of micronuclei in squamous epithelial cells of human oral mucosa.
MedLine Citation:
PMID:  9893901     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the influence of methodologic variables, staining method and sampling, on the frequency of micronuclei scored in squamous epithelial cells of oral mucosa. Micronuclei were used as biomarkers of structural and numerical chromosome damage.
STUDY DESIGN: Feulgen and Giemsa stain and fluorescent dyes Hoechst 33258 and propidium iodide were used for micronucleus staining. Cells were collected by either light or vigorous scraping of oral mucosa.
RESULTS: Staining of micronuclei by Hoechst 33258 proved more handly, less time consuming and at least equally reliable to the more widely used Feulgen staining. Results with Giemsa stain and propidium iodide were unsatisfactory. Micronuclei were less frequent in superficial layers of the oral mucosa.
CONCLUSION: Hoechst 33258 can be recommended for micronucleus staining in squamous epithelial cells of oral mucosa. Differences in scraping may affect the measurement of micronucleus frequency; scraping should therefore be carefully standardized in each laboratory.
G Casartelli; S Monteghirfo; M De Ferrari; S Bonatti; M Scala; S Toma; G Margarino; A Abbondandolo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology     Volume:  19     ISSN:  0884-6812     ISO Abbreviation:  Anal. Quant. Cytol. Histol.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1999-04-13     Completed Date:  1999-04-13     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  8506819     Medline TA:  Anal Quant Cytol Histol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  475-81     Citation Subset:  IM    
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MeSH Terms
Epithelial Cells / cytology
Micronuclei, Chromosome-Defective*
Mouth Mucosa / cytology*
Staining and Labeling / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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