| Staining of human pituitary glands with lead hematoxylin in comparison with other histochemical procedures, including the immunoenzyme technique. | |
| | |
MedLine Citation:
|
PMID: 411317 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Human pituitary glands and pituitary adenomas, obtained by surgery or at autopsy, have been stained with lead hematoxylin and the results were compared with those of other histochemical procedures, including the immunoperoxidase technique. Lead hematoxylin positivity was found in the cytoplasm of melanocorticotroph, thyrotroph as well as gonadotroph cells, indicating that lead hematoxylin has no selective affinity for melanocorticotroph cells. Lead hematoxylin positive cells also stained with the PAS technique. Lead hematoxylin positivity was not revealed in growth hormone cells or in prolactin cells. Basophilic adenomas, composed of melanocorticotroph cells and associated with Cushing's disease or with Nelson's syndrome or unaccompanied by hormonal hypersecretion, showed intense lead hematoxylin positivity. Thus, no correlation was evident between lead hematoxylin positivity and secretory activity. Acidophilic or chromophobic adenomas consisting of growth hormone cells, prolactin cells, stem cells, undifferentiated cells or oncocytes, however, were negative. It can be concluded that the lead hematoxylin staining techniques a reliable, easy and reproducible histochemical method, and its use can be recommended in studies related to adenohypophysial cytology. |
| | |
Authors:
|
N Ryan; K Kovacs; C Ezrin |
Related Documents
:
|
8119197 - Autocrine interaction between prolactin and its receptor occurs intracellularly in the ... 229957 - Morphological changes of pituitary gonadotrophs and thyrotrophs following treatment wit... 19937347 - Cell organization of the rat pars tuberalis. evidence for open communication between pa... 11741317 - Gnrh agonist buserelin affects colony-forming efficiency of hhua and jurkat cells. 1378857 - Hemopoietic growth factors: a review. 1499857 - Variation in sorbitol accumulation and polyol-pathway activity in cultured human proxim... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Acta histochemica Volume: 59 ISSN: 0065-1281 ISO Abbreviation: Acta Histochem. Publication Date: 1977 |
Date Detail:
|
Created Date: 1977-12-29 Completed Date: 1977-12-29 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0370320 Medline TA: Acta Histochem Country: GERMANY, EAST |
Other Details:
|
Languages: eng Pagination: 96-105 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenoma
/
pathology* Hematoxylin Histocytochemistry Humans Immunoenzyme Techniques Lead Pituitary Gland / cytology*, pathology Pituitary Neoplasms / pathology* |
| Chemical | |
Reg. No./Substance:
|
517-28-2/Hematoxylin; 7439-92-1/Lead |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Immunohistochemical staining using coupled immunofluorescence and immunocomplex technique.
Next Document: One-sided gingival hyperplasia after treatment with diphenylhydantoin.