| Staining of fungal cell walls with fluorescent brighteners: flow-cytometric analysis. | |
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MedLine Citation:
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PMID: 1702746 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Spore walls of Backusella lamprospora (Mucorales) were stained with ten fluorescent brighteners (FB) and the intensity of their fluorescence was determined. The fluorescence was most intense with Uvitex 2B (100%), other brighteners yielding lower fluorescence intensities: Blankophor BA 267% and BA 200% about 75%, Rylux BSU about 50%, other Rylux agents 10-30%. The agents most suitable for microscopic diagnostics of human and animal mycoses are Uvitex 2B, Blankophor BA 267% and BA 200%, Rylux BSU, and also Rylux BS and PRS. The regulation of excessive fluorescence of fungal cells during microscopic observation is discussed. For the purposes of microscopic diagnosis of human and animal mycosis Uvitex 2B, Blankophor BA 267% and BA 200%, Rylux BSU and, possibly, Rylux BS and PRS are recommended. |
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Authors:
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M Hejtmánek; J Dolezel; I Holubová |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Folia microbiologica Volume: 35 ISSN: 0015-5632 ISO Abbreviation: Folia Microbiol. (Praha) Publication Date: 1990 |
Date Detail:
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Created Date: 1991-02-19 Completed Date: 1991-02-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376757 Medline TA: Folia Microbiol (Praha) Country: CZECHOSLOVAKIA |
Other Details:
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Languages: eng Pagination: 437-42 Citation Subset: IM |
Affiliation:
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Department of Medical Biology, Faculty of Medicine, Palacký University, Olomouc, Czechoslovakia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Wall / chemistry Flow Cytometry* Fluorescent Dyes* Humans Mucorales / isolation & purification* Mycoses / diagnosis* Spores, Fungal / isolation & purification Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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