Document Detail


Staining of fungal cell walls with fluorescent brighteners: flow-cytometric analysis.
MedLine Citation:
PMID:  1702746     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Spore walls of Backusella lamprospora (Mucorales) were stained with ten fluorescent brighteners (FB) and the intensity of their fluorescence was determined. The fluorescence was most intense with Uvitex 2B (100%), other brighteners yielding lower fluorescence intensities: Blankophor BA 267% and BA 200% about 75%, Rylux BSU about 50%, other Rylux agents 10-30%. The agents most suitable for microscopic diagnostics of human and animal mycoses are Uvitex 2B, Blankophor BA 267% and BA 200%, Rylux BSU, and also Rylux BS and PRS. The regulation of excessive fluorescence of fungal cells during microscopic observation is discussed. For the purposes of microscopic diagnosis of human and animal mycosis Uvitex 2B, Blankophor BA 267% and BA 200%, Rylux BSU and, possibly, Rylux BS and PRS are recommended.
Authors:
M Hejtmánek; J Dolezel; I Holubová
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Folia microbiologica     Volume:  35     ISSN:  0015-5632     ISO Abbreviation:  Folia Microbiol. (Praha)     Publication Date:  1990  
Date Detail:
Created Date:  1991-02-19     Completed Date:  1991-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376757     Medline TA:  Folia Microbiol (Praha)     Country:  CZECHOSLOVAKIA    
Other Details:
Languages:  eng     Pagination:  437-42     Citation Subset:  IM    
Affiliation:
Department of Medical Biology, Faculty of Medicine, Palacký University, Olomouc, Czechoslovakia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Wall / chemistry
Flow Cytometry*
Fluorescent Dyes*
Humans
Mucorales / isolation & purification*
Mycoses / diagnosis*
Spores, Fungal / isolation & purification
Staining and Labeling
Chemical
Reg. No./Substance:
0/Fluorescent Dyes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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