Document Detail


Staining of central neurons of the pteropod mollusk Clione limacina with fluorescein-labeled alpha-bungarotoxin.
MedLine Citation:
PMID:  10493544     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fluorescein-labeled alpha-bungarotoxin (alphaBT/FITC) was used to identify neurons bearing acetylcholine receptors in the central nervous system of the pteropod mollusk Clione limacina. AlphaBT/FITC stained two small symmetrical neurons on the dorsal surface of the cerebral ganglia. The stain was highly specific and was completely blocked by preincubation with a very low concentration of unlabeled alpha-bungarotoxin (1 nM). Nicotinic receptor agonists and antagonists effectively blocked staining with alphaBT/FITC, while muscarinic agonists and antagonists had no effect. This provides evidence that alphaBT/FITC genuinely binds to acetylcholine receptors. The possible role of the alphaBT/FITC-stained cells in the hunting behavioral complex of Clione is discussed.
Authors:
L B Popova; Panchin YuV
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience and behavioral physiology     Volume:  29     ISSN:  0097-0549     ISO Abbreviation:  Neurosci. Behav. Physiol.     Publication Date:    1999 May-Jun
Date Detail:
Created Date:  1999-10-19     Completed Date:  1999-10-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0330471     Medline TA:  Neurosci Behav Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  317-20     Citation Subset:  IM    
Affiliation:
Institute of Information Transmission Problems, Russian Academy of Sciences, Moscow.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bungarotoxins*
Central Nervous System / cytology,  physiology*
Coloring Agents
Fluoresceins*
Ganglia, Invertebrate / cytology,  physiology
Neurons / physiology*
Nicotinic Agonists / pharmacology
Nicotinic Antagonists / pharmacology
Receptors, Nicotinic / drug effects
Snails / physiology*
Chemical
Reg. No./Substance:
0/Bungarotoxins; 0/Coloring Agents; 0/Fluoresceins; 0/Nicotinic Agonists; 0/Nicotinic Antagonists; 0/Receptors, Nicotinic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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