Document Detail

Stage-specificity of spontaneous mutation at a tandem repeat DNA locus in the mouse germline.
MedLine Citation:
PMID:  18423770     Owner:  NLM     Status:  MEDLINE    
Mouse expanded simple tandem repeat (ESTR) loci are the most unstable loci in the mouse genome. Despite the fact that over the last decade these loci have been extensively used for studying germline mutation induction in mice, to date little is known about the mechanisms underlying spontaneous and induced ESTR mutation. Here we used flow cytometry and single-molecule PCR to compare the frequency of ESTR mutation in four flow-sorted fractions of the mouse male germ cells - spermatogonia, spermatocytes I, round and elongated spermatids. The frequency and the spectrum of ESTR mutation did not significantly differ between different stages of mouse spermatogenesis. Considering these data and the results of other publications, we propose that spontaneous ESTR mutation is mostly attributed to replication slippage in spermatogonia and these loci may be regarded as a class of expanded microsatellites.
Morag Shanks; Lydia Riou; Pierre Fouchet; Yuri E Dubrova
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-03-26
Journal Detail:
Title:  Mutation research     Volume:  641     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-29     Completed Date:  2008-07-08     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  58-60     Citation Subset:  IM    
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MeSH Terms
Flow Cytometry
Germ-Line Mutation / genetics*
Mice, Inbred CBA
Polymerase Chain Reaction
Spermatids / physiology*
Spermatocytes / cytology,  metabolism
Spermatogenesis / genetics*
Spermatogonia / cytology,  physiology
Tandem Repeat Sequences / genetics*
Grant Support
G0300477//Medical Research Council; //Medical Research Council

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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