Document Detail


Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.
MedLine Citation:
PMID:  23301556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One important challenge for regenerative medicine is to produce a clinically relevant number of cells with consistent tissue-forming potential. Isolation and expansion of cells from skeletal tissues results in a heterogeneous population of cells with variable regenerative potential. A more consistent tissue formation could be achieved by identification and selection of potent progenitors based on cell surface molecules. In this study, we assessed the expression of stage-specific embryonic antigen-4 (SSEA-4), a classic marker of undifferentiated stem cells, and other surface markers in human articular chondrocytes (hACs), osteoblasts, and bone marrow-derived mesenchymal stromal cells (bmMSCs) and characterized their differentiation potential. Further, we sorted SSEA-4-expressing hACs and followed their potential to proliferate and to form cartilage in vitro. Cells isolated from cartilage and bone exhibited remarkably heterogeneous SSEA-4 expression profiles in expansion cultures. SSEA-4 expression levels increased up to ∼5 population doublings, but decreased following further expansion and differentiation cultures; levels were not related to the proliferation state of the cells. Although SSEA-4-sorted chondrocytes showed a slightly better chondrogenic potential than their SSEA-4-negative counterparts, differences were insufficient to establish a link between SSEA-4 expression and chondrogenic potential. SSEA-4 levels in bmMSCs also did not correlate to the cells' chondrogenic and osteogenic potential in vitro. SSEA-4 is clearly expressed by subpopulations of proliferating somatic cells with a MSC-like phenotype. However, the predictive value of SSEA-4 as a specific marker of superior differentiation capacity in progenitor cell populations from adult human tissue and even its usefulness as a stem cell marker appears questionable.
Authors:
Karsten Schrobback; Jana Wrobel; Dietmar W Hutmacher; Tim B F Woodfield; Travis J Klein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-02-19
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  19     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-04-29     Completed Date:  2013-11-15     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1316-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aged
Biological Markers / metabolism
Bone Marrow Cells / cytology,  metabolism
Cartilage, Articular / cytology
Cell Proliferation
Cells, Cultured
Chondrocytes / cytology,  metabolism*
Chondrogenesis*
Female
Flow Cytometry
Humans
Male
Mesenchymal Stromal Cells / cytology,  metabolism
Middle Aged
Osteoblasts / cytology,  metabolism
Osteogenesis*
Stage-Specific Embryonic Antigens / metabolism*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Stage-Specific Embryonic Antigens; 0/stage-specific embryonic antigen-4
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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