Document Detail

Stage-specific differences in the requirements for germline stem cell maintenance in the Drosophila ovary.
MedLine Citation:
PMID:  18213359     Owner:  NLM     Status:  MEDLINE    
In this study, we uncover a role for microRNAs in Drosophila germline stem cell (GSC) maintenance. Disruption of Dicer-1 function in GSCs during adult life results in GSC loss. Surprisingly, however, loss of Dicer-1 during development does not result in a GSC maintenance defect, although a defect is seen if both Dicer-1 and Dicer-2 function are disrupted. Loss of the bantam microRNA mimics the Dicer-1 maintenance defect when induced in adult GSCs, suggesting that bantam plays a key role in GSC self-renewal. Mad, a component of the TGF-beta pathway, behaves similarly to Dicer-1: adult GSC maintenance requires Mad if it is lost during adult life, but not if it is lost during pupal development. Overall, these results show stage-specific differential sensitivity of GSC maintenance to certain perturbations, and suggest that there may be Dcr-2 dependent redundancy of GSC maintenance mechanisms during development that is lost in later life.
Halyna R Shcherbata; Ellen J Ward; Karin A Fischer; Jenn-Yah Yu; Steven H Reynolds; Chun-Hong Chen; Peizhang Xu; Bruce A Hay; Hannele Ruohola-Baker
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell stem cell     Volume:  1     ISSN:  1875-9777     ISO Abbreviation:  Cell Stem Cell     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2008-03-28     Completed Date:  2008-08-18     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  101311472     Medline TA:  Cell Stem Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  698-709     Citation Subset:  IM    
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MeSH Terms
Drosophila / cytology*,  genetics*
Drosophila Proteins / genetics*
G1 Phase
Germ Cells / cytology*,  metabolism*
MicroRNAs / genetics,  physiology*
Ovary / cytology*
RNA Helicases / genetics*
Ribonuclease III
S Phase
Signal Transduction
Grant Support
R01 GM062748/GM/NIGMS NIH HHS; R01 GM062748-10/GM/NIGMS NIH HHS; R01 GM062748-11/GM/NIGMS NIH HHS; R01 GM083867/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Drosophila Proteins; 0/MicroRNAs; EC 2.7.7.-/DICER-1 protein, Drosophila; EC III; EC Helicases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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