Document Detail


Stage-specific damage to synaptonemal complexes and metaphase chromosomes induced by X rays in male mouse germ cells.
MedLine Citation:
PMID:  1996377     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Synaptonemal complexes reveal mutagen-induced effects in germ cell meiotic chromosomes. This study was aimed at characterizing relationships between damage to synaptonemal complexes and metaphase I chromosomes following radiation exposure at various stages of spermatogenesis. Male mice were irradiated with doses of 0, 2, or 4 Gy, and spermatocytes were harvested at times consistent with earlier exposures as spermatogonial stem cells, preleptotene cells (premeiotic DNA synthesis), or meiotic prophase cells. After stem-cell exposure, twice as many rearrangements were observed in synaptonemal complexes as in metaphase I chromosomes. Irradiation during premeiotic DNA synthesis resulted in dose-related increases in synaptonemal complex breakage and rearrangements (including novel forms) and in metaphase chromosomal aberrations. Following prophase exposure, various types and levels of damage to synaptonemal complexes and metaphase chromosomes were observed. Irradiation of zygotene cells led to high frequencies of chromosome multivalents in metaphase I without a correspondingly high level of damage in preceding prophase synaptonemal complexes. Thus irradiation of premeiotic and meiotic cells results in variable relationships between damage to synaptonemal complexes and metaphase chromosomes. Interpretations of these relationships are based upon what is known about both radiation clastogenesis and the structural/temporal relationships between synaptonemal complexes at prophase and chromosomes at metaphase I of meiosis.
Authors:
L C Backer; M R Sontag; J W Allen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Radiation research     Volume:  125     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  1991 Feb 
Date Detail:
Created Date:  1991-03-25     Completed Date:  1991-03-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  187-96     Citation Subset:  IM; S    
Affiliation:
Environmental Health Research and Testing, Research Triangle Park, North Carolina 27709.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosome Aberrations
Chromosomes / radiation effects*
Male
Metaphase
Mice
Mice, Inbred C57BL
Spermatocytes / cytology,  radiation effects*
Synaptonemal Complex / radiation effects*

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