Document Detail


Stably transformed insect cell lines: tools for expression of secreted and membrane-anchored proteins and high-throughput screening platforms for drug and insecticide discovery.
MedLine Citation:
PMID:  16997011     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Insect cell-based expression systems are prominent amongst current expression platforms for their ability to express virtually all types of heterologous recombinant proteins. Stably transformed insect cell lines represent an attractive alternative to the baculovirus expression system, particularly for the production of secreted and membrane-anchored proteins. For this reason, transformed insect cell systems are receiving increased attention from the research community and the biotechnology industry. In this article, we review recent developments in the field of insect cell-based expression from two main perspectives, the production of secreted and membrane-anchored proteins and the establishment of novel methodological tools for the identification of bioactive compounds that can be used as research reagents and leads for new pharmaceuticals and insecticides.
Authors:
Vassilis Douris; Luc Swevers; Vassiliki Labropoulou; Evi Andronopoulou; Zafiroula Georgoussi; Kostas Iatrou
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Advances in virus research     Volume:  68     ISSN:  0065-3527     ISO Abbreviation:  Adv. Virus Res.     Publication Date:  2006  
Date Detail:
Created Date:  2006-09-25     Completed Date:  2006-11-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370441     Medline TA:  Adv Virus Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  113-56     Citation Subset:  IM    
Affiliation:
Insect Molecular Genetics and Biotechnology Group, Institute of Biology National Centre for Scientific Research Demokritos, GR 153 10 Aghia Paraskevi Attikis (Athens), Greece.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Transformed
Drug Design*
Enhancer Elements, Genetic
Insecticides*
Insects / genetics*
Membrane Proteins / biosynthesis*
Polyadenylation
Promoter Regions, Genetic
Receptors, Cytoplasmic and Nuclear / biosynthesis
Receptors, G-Protein-Coupled / biosynthesis
Recombinant Proteins / biosynthesis*
Trans-Activators / physiology
Chemical
Reg. No./Substance:
0/Insecticides; 0/Membrane Proteins; 0/Receptors, Cytoplasmic and Nuclear; 0/Receptors, G-Protein-Coupled; 0/Recombinant Proteins; 0/Trans-Activators

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