| Stably transformed insect cell lines: tools for expression of secreted and membrane-anchored proteins and high-throughput screening platforms for drug and insecticide discovery. | |
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MedLine Citation:
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PMID: 16997011 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Insect cell-based expression systems are prominent amongst current expression platforms for their ability to express virtually all types of heterologous recombinant proteins. Stably transformed insect cell lines represent an attractive alternative to the baculovirus expression system, particularly for the production of secreted and membrane-anchored proteins. For this reason, transformed insect cell systems are receiving increased attention from the research community and the biotechnology industry. In this article, we review recent developments in the field of insect cell-based expression from two main perspectives, the production of secreted and membrane-anchored proteins and the establishment of novel methodological tools for the identification of bioactive compounds that can be used as research reagents and leads for new pharmaceuticals and insecticides. |
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Authors:
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Vassilis Douris; Luc Swevers; Vassiliki Labropoulou; Evi Andronopoulou; Zafiroula Georgoussi; Kostas Iatrou |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Advances in virus research Volume: 68 ISSN: 0065-3527 ISO Abbreviation: Adv. Virus Res. Publication Date: 2006 |
Date Detail:
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Created Date: 2006-09-25 Completed Date: 2006-11-03 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370441 Medline TA: Adv Virus Res Country: United States |
Other Details:
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Languages: eng Pagination: 113-56 Citation Subset: IM |
Affiliation:
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Insect Molecular Genetics and Biotechnology Group, Institute of Biology National Centre for Scientific Research Demokritos, GR 153 10 Aghia Paraskevi Attikis (Athens), Greece. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line, Transformed Drug Design* Enhancer Elements, Genetic Insecticides* Insects / genetics* Membrane Proteins / biosynthesis* Polyadenylation Promoter Regions, Genetic Receptors, Cytoplasmic and Nuclear / biosynthesis Receptors, G-Protein-Coupled / biosynthesis Recombinant Proteins / biosynthesis* Trans-Activators / physiology |
| Chemical | |
Reg. No./Substance:
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0/Insecticides; 0/Membrane Proteins; 0/Receptors, Cytoplasmic and Nuclear; 0/Receptors, G-Protein-Coupled; 0/Recombinant Proteins; 0/Trans-Activators |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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