| Stable transformation of mature zygotic embryos and regeneration of transgenic plants of chir pine (Pinus roxbughii Sarg.). | |
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MedLine Citation:
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PMID: 16133348 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A particle inflow gun was used to transfer the plasmid pAHC25 containing the bar gene conferring resistance to glufosinate and the gusA reporter gene, each driven by the maize ubiquitin promoter, to mature embryos of Pinus roxburghii (chir pine). High levels of transient expression were obtained when embryos were cultured for 6 days on 10 microM benzyl adenine-containing medium and then exposed to high osmoticum (0.5 M sucrose) before and after bombardment. Selection on medium containing Basta enabled recovery of stably transformed shoots, both from the epicotyl and from adventitious buds. The primary transformed shoots from the epicotyl were multiplied via axillary shoots. Transformation was confirmed by histochemical staining for beta-glucuronidase (GUS) activity, by polymerase chain reaction (PCR) amplification of fragments of gusA and nos terminator, and by the resistance of needles to Basta. |
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Authors:
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V A Parasharami; V B Naik; S von Arnold; R S Nadgauda; D H Clapham |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-08-17 |
Journal Detail:
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Title: Plant cell reports Volume: 24 ISSN: 0721-7714 ISO Abbreviation: Plant Cell Rep. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-01-06 Completed Date: 2006-05-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9880970 Medline TA: Plant Cell Rep Country: Germany |
Other Details:
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Languages: eng Pagination: 708-14 Citation Subset: IM |
Affiliation:
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Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, Pune, 411 008, Maharashtra, India. varshapp2@yahoo.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Biolistics DNA Primers DNA, Plant Pinus / embryology, genetics, physiology* Plants, Genetically Modified / embryology, genetics, physiology* Plasmids Polymerase Chain Reaction Seeds / growth & development* Transformation, Genetic* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Plant |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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