Document Detail


Stable polyphosphate accumulation by a pseudo-revertant of an Escherichia coli phoU mutant.
MedLine Citation:
PMID:  23288295     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
phoU mutants of bacteria are potentially useful for the removal of inorganic phosphate (P(i)) from sewage because they can accumulate a large amounts of polyphosphate (polyP). However, the growth of phoU mutants is severely defective and is easily outgrown by revertant(s) that have lost the ability to accumulate polyP during growth in a nutrient-rich medium. We found that a pseudo-revertant, designated LAP[+], that appeared in a culture of an Escherichia coli phoU mutant that could accumulate polyP even after ten serial passages. Reduction in the expression of the P(i)-specific transporter Pst in LAP[+] may contribute to relieving stresses such as excess P(i) incorporation that could stimulate reversions. The discovery of a LAP[+] provides a clue to generate phoU mutants that accumulate polyP in a stable manner.
Authors:
Ryuichi Hirota; Kei Motomura; Shigeto Nakai; Tomohiro Handa; Takeshi Ikeda; Akio Kuroda
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-4
Journal Detail:
Title:  Biotechnology letters     Volume:  -     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima, 739-8530, Japan, hirota@hiroshima-u.ac.jp.
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