Document Detail


Stable isotopic abundances as reflections of ingestion practices.
MedLine Citation:
PMID:  6518786     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The proportions of stable isotopes found in biological tissues (e.g., 13C/12C, 15N/14N, or 18O/16O) are constant only on a macroscopic scale. Sensitive mass spectrometric measurements of the isotope ratio or isotopic abundance for each element show that the tissue (and organism) displays small excursions in these abundances, which are reflections of isotope fractionations that occur in prior stages of the food chain. These excursions can be detected in respiratory CO2 in urine, and in stool, with changes over time intervals as short as 2 hours or as long as 6-12 months. Perturbation of these stable isotopic abundances has been used to demonstrate phenomena as gross as the change in carbon sources of milk proteins when cows are moved from winter silage to summer pastures, or as subtle as the utilization of a single feeding of complex carbohydrates by infants by measuring the isotopic abundance of their respiratory CO2. The use of marker molecules enriched with 13C, 15N, or 18O thus provides a nonpharmacological, nonradioactive, and under ideal conditions, noninvasive procedure to document the ingestion of single or multiple doses of the pharmacological agent being tested. Such generic markers are potentially suitable for all study populations, regardless of developmental age, reproductive activity, or degree of compliance.
Authors:
P D Klein
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Controlled clinical trials     Volume:  5     ISSN:  0197-2456     ISO Abbreviation:  Control Clin Trials     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1985-03-13     Completed Date:  1985-03-13     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8006242     Medline TA:  Control Clin Trials     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  568-72     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Breath Tests
Carbon Isotopes / analysis
Deuterium / analysis
Feces / analysis
Humans
Isotopes / analysis*
Nitrogen Isotopes / analysis
Oxygen Isotopes / analysis
Patient Compliance*
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Isotopes; 0/Nitrogen Isotopes; 0/Oxygen Isotopes; 7782-39-0/Deuterium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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