Document Detail

Stable isotope tracer and gas-chromatography combustion isotope ratio mass spectrometry to study the in vivo compartmental metabolism of docosahexaenoic acid.
MedLine Citation:
PMID:  7978245     Owner:  NLM     Status:  MEDLINE    
A gas-chromatography combustion isotope ratio mass spectrometry (GCC-IRMS) method using carbon 13 (13C)-stable isotope to trace n-3 polyunsaturated fatty acids (PUFA) turnover in vivo is presented. Natural 13C abundance of commercial n-3 PUFA was measured from 100 to 300 ng of fatty acids and was -27.58, -27.83, and -28.16 for 22:6n-3, 22:5n-3, and 20:5n-3, expressed as delta 13C /1000 versus Pee Dee Belemnite (PDB), respectively. Precision of delta 13C /1000 values was comparable for the three PUFA and gave relative standard deviations of 0.95-0.97%. Isotope enrichment of 0.0010 at.% could be detected. Triglycerides enriched in [13C]22:6n-3 ([13C]22:6-TG) were synthesized by growing a microalgae on [1-13C]glucose. [13C]22:6n-3 represented 36 wt.% of total triglyceride fatty acids and had an isotope enrichment of 2.0420 at.%, which was the double of natural abundance. The isotope enrichment of 22:6n-3 in lipids from rat lipoproteins and red cells could be followed as a function of time after ingestion of 3 mg [13C]22:6-TG and showed specific patterns according to the lipid compartments. The retroconversion of [13C]22:6n-3 was also detected in HDL phosphatidylcholine by the appearance of [13C]22:5n-3 and [13C]20:5n-3. On the other hand, 22:6n-3 natural 13C abundance in human lipid classes of lipoproteins and blood cells has been measured using 10 ml plasma, even for the more limiting lipid compartments in terms of 22:6n-3 dose size.(ABSTRACT TRUNCATED AT 250 WORDS)
N Brossard; C Pachiaudi; M Croset; S Normand; J Lecerf; V Chirouze; J P Riou; J L Tayot; M Lagarde
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  220     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-12-12     Completed Date:  1994-12-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  192-9     Citation Subset:  IM    
INSERM U 352, Chimie Biologique, INSA-Lyon, Villeurbanne, France.
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MeSH Terms
Carbon Isotopes
Docosahexaenoic Acids / blood,  metabolism*
Gas Chromatography-Mass Spectrometry / methods*
Lipid Metabolism*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Carbon Isotopes; 25167-62-8/Docosahexaenoic Acids

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