| Stable isotope ratio analysis as a tool to discriminate between rainbow trout (O. mykiss) fed diets based on plant or fish-meal proteins. | |
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MedLine Citation:
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PMID: 18973201 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The use of stable isotope ratio analysis (SIRA) as a rapid analytical tool to characterize and discriminate farmed fish on the basis of the feedstuffs included in the diet formulation is discussed. Two isoproteic (44.8%) and isolipidic (19.6%) extruded diets were formulated: a fish-meal-based diet (FM diet), containing fish meal as the sole protein source; a plant-protein-based diet (PP diet), where pea protein concentrate and wheat gluten meal replaced 80% of fish meal protein. The diets were fed to eight groups of rainbow trout (initial body weight: 106.6g) for 103 days in two daily meals under controlled rearing conditions. Growth performance (final body weight: 318.5 g; specific growth rate: 1.06%) and feed-to-gain ratio (0.79) were not affected by the dietary treatment. The differences in isotopic values of the two diets were clearly reflected in the different carbon and nitrogen isotopic values in rainbow trout fillets. The delta(13)C and delta(15)N values of muscle of farmed rainbow trout showed differences between farmed fish fed a fish-protein-based diet (-20.47 +/- 0.34 and 12.38 +/- 0.57 for delta(13)C and delta(15)N, respectively) and those fed a plant-protein-based diet (-23.96 +/- 0.38 and 7.15 +/- 0.51 for delta(13)C and delta(15)N, respectively). The results suggest that SIRA provides a robust and verifiable analytical tool to discriminate between fish fed on a plant or a fish protein diet. |
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Authors:
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J M Moreno-Rojas; F Tulli; M Messina; E Tibaldi; C Guillou |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Rapid communications in mass spectrometry : RCM Volume: 22 ISSN: 0951-4198 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-17 Completed Date: 2009-05-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
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Languages: eng Pagination: 3706-10 Citation Subset: IM |
Affiliation:
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Institute for Health and Consumer Protection, Physical and Chemical Exposure Unit, MAST-Group, TP281, Via Fermi 2, 21020 Ispra (VA), Italy. jose-manuel.moreno-rojas@jrc.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed* Animals Carbon Isotopes / analysis* Diet Dietary Proteins / analysis Fish Products / analysis Fish Proteins / administration & dosage*, chemistry Glutens / chemistry Mass Spectrometry Nitrogen Isotopes / analysis* Oncorhynchus mykiss / growth & development*, metabolism Peas / chemistry Plant Proteins / administration & dosage*, chemistry Triticum / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Dietary Proteins; 0/Fish Proteins; 0/Nitrogen Isotopes; 0/Plant Proteins; 8002-80-0/Glutens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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