| Stable isotope probing - linking microbial identity to function. | |
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MedLine Citation:
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PMID: 15886694 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as (13)C, and the identification of active microorganisms by the selective recovery and analysis of isotope-enriched cellular components. DNA and rRNA are the most informative taxonomic biomarkers and (13)C-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation. The future holds great promise for SIP, particularly when combined with other emerging technologies such as microarrays and metagenomics. |
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Authors:
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Marc G Dumont; J Colin Murrell |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Nature reviews. Microbiology Volume: 3 ISSN: 1740-1526 ISO Abbreviation: Nat. Rev. Microbiol. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-06-02 Completed Date: 2005-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101190261 Medline TA: Nat Rev Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 499-504 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteria
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classification,
genetics* Carbon Isotopes DNA, Bacterial / genetics* Isotope Labeling / methods Phylogeny Polymerase Chain Reaction / methods RNA, Ribosomal, 16S / genetics |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/DNA, Bacterial; 0/RNA, Ribosomal, 16S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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