Document Detail


Stable isotope probing and Raman spectroscopy for monitoring carbon flow in a food chain and revealing metabolic pathway.
MedLine Citation:
PMID:  23259452     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Accurately measuring carbon flows is a challenge for understanding processes such as diverse intracellular metabolic pathways and foodwebs in ecosystem. Combined with stable isotope probing (SIP), single-cell Raman spectroscopy was demonstrated for the first time to link the food chain from carbon substrate to bacterial prey up to predators at single-cell level in a quantitative and non-destructive manner. Escherichia coli OP50 with different 13C content, which were grown in a mixture of 12C- and fully carbon-labelled 13C-glucose (99%) as sole carbon source, were fed to Caenorhabditis elegans. The E. coli grazer - C. elegans was observed to be 13C-labelled at levels dependent on 13C-content of the fed E. coli cells. Two Raman spectral biomarkers (Raman bands for phenylalanine at 1001 cm-1 and thymine at 747 cm-1 Raman bands), were used to quantify the 13C-content in E. coli and C. elegans over a range of 1.1-99%. The phenylalanine Raman band was a suitable biomarker for prokaryotic cells and thymine Raman band for eukaryotic cells. A biochemical mechanism accounting for the Raman red-shifts of phenylalanine and thymine in response to 13C-labelling is proposed in this study and is supported by quantum chemical calculation. This study offers new insights of carbon flow via food-chain, and provides a research tool for microbial ecology and investigation of biochemical pathways.
Authors:
Mengqiu Li; Wei E Huang; Christopher M Gibson; Patrick William Fowler; Alexandre Jousset
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-22
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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