| Stable isotope probing and Raman spectroscopy for monitoring carbon flow in a food chain and revealing metabolic pathway. | |
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MedLine Citation:
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PMID: 23259452 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Accurately measuring carbon flows is a challenge for understanding processes such as diverse intracellular metabolic pathways and foodwebs in ecosystem. Combined with stable isotope probing (SIP), single-cell Raman spectroscopy was demonstrated for the first time to link the food chain from carbon substrate to bacterial prey up to predators at single-cell level in a quantitative and non-destructive manner. Escherichia coli OP50 with different 13C content, which were grown in a mixture of 12C- and fully carbon-labelled 13C-glucose (99%) as sole carbon source, were fed to Caenorhabditis elegans. The E. coli grazer - C. elegans was observed to be 13C-labelled at levels dependent on 13C-content of the fed E. coli cells. Two Raman spectral biomarkers (Raman bands for phenylalanine at 1001 cm-1 and thymine at 747 cm-1 Raman bands), were used to quantify the 13C-content in E. coli and C. elegans over a range of 1.1-99%. The phenylalanine Raman band was a suitable biomarker for prokaryotic cells and thymine Raman band for eukaryotic cells. A biochemical mechanism accounting for the Raman red-shifts of phenylalanine and thymine in response to 13C-labelling is proposed in this study and is supported by quantum chemical calculation. This study offers new insights of carbon flow via food-chain, and provides a research tool for microbial ecology and investigation of biochemical pathways. |
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Authors:
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Mengqiu Li; Wei E Huang; Christopher M Gibson; Patrick William Fowler; Alexandre Jousset |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-22 |
Journal Detail:
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Title: Analytical chemistry Volume: - ISSN: 1520-6882 ISO Abbreviation: Anal. Chem. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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