| Stable isotope labeling of entire Bacillus atrophaeus spores and vegetative cells using bioaerosol mass spectrometry. | |
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MedLine Citation:
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PMID: 15858989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Single vegetative cells and spores of Bacillus atrophaeus, formerly Bacillus subtilis var. niger, were analyzed using bioaerosol mass spectrometry. Key biomarkers were identified from organisms grown in 13C and 15N isotopically enriched media. Spore spectra contain peaks from dicipolinate and amino acids. The results indicate that compounds observed in the spectra correspond to material from the spore's core and not the exosporium. Standard compounds and mixtures were analyzed for comparison. The biomarkers for vegetative cells were clearly different from those of the spores, consisting mainly of phosphate clusters and amino acid fragments. |
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Authors:
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Gregg A Czerwieniec; Scott C Russell; Herbert J Tobias; Maurice E Pitesky; David P Fergenson; Paul Steele; Abneesh Srivastava; Joanne M Horn; Matthias Frank; Eric E Gard; Carlito B Lebrilla |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Analytical chemistry Volume: 77 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-04-29 Completed Date: 2007-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1081-7 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of California, Davis, California 95616, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aerosols Amino Acids / analysis Bacillus subtilis / chemistry* Biological Markers Isotope Labeling* Mass Spectrometry / methods* Spores, Bacterial / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Aerosols; 0/Amino Acids; 0/Biological Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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