Document Detail

Stable isotope analysis at the molecular level: a new approach for determining the origins of amino acids in the Murchison meteorite.
MedLine Citation:
PMID:  11539266     Owner:  NASA     Status:  MEDLINE    
A combined gas chromatography/isotope ratio mass spectrometry (GC/IRMS) method has been developed that permits the direct stable carbon isotope analysis of N(O)-trifluoroacetyl-isopropyl esters of individual amino acids and their respective enantiomers at nanomole abundances. Calculation of the original delta 13C values of the amino acids is accomplished via a correction for the carbon introduced during the derivatization process. Previous GC/IRMS analyses of individual amino acids in the non-hydrolyzed water extract of an interior sample of a Murchison meteorite stone revealed an enrichment in 13C relative to terrestrial organic matter, in agreement with previous findings for bulk extracts. The range of amino acid delta 13C values (+5 to +30%, PDB) suggests possible kinetic effects during synthesis. In this study, an apparent kinetic isotope effect was also observed for the amino acid products of a spark discharge experiment. These preliminary results are supportive of a similar mechanism for the abiotic synthesis of amino acids in the Murchison meteorite.
M H Engel; S A Macko; Y Qian; J A Silfer
Related Documents :
11976926 - Dehydration modifies guanidino compound concentrations in the different zones of the ra...
6783106 - Metabolism of odd-numbered, normal fatty acids in tetrahymena pyriformis w.
7623186 - Topical application of 5-aminolevulinic acid, dmso and edta: protoporphyrin ix accumula...
749926 - Erythrocyte delta-aminolaevulinic acid dehydratase activity and changes in delta-aminol...
7372546 - Studies on new phosphonic acid antibiotics. ii. taxonomic studies on producing organism...
20306516 - Relative acidities and structure analysis of cis and trans isomers of 1,5-oxazaspiro[5....
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Advances in space research : the official journal of the Committee on Space Research (COSPAR)     Volume:  15     ISSN:  0273-1177     ISO Abbreviation:  Adv Space Res     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1997-03-21     Completed Date:  1997-03-21     Revised Date:  2007-07-17    
Medline Journal Info:
Nlm Unique ID:  9878935     Medline TA:  Adv Space Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  99-106     Citation Subset:  S    
School of Geology and Geophysics, The University of Oklahoma, Norman 73019, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Alanine / analysis,  chemical synthesis
Amino Acids / analysis,  chemical synthesis*
Aminoisobutyric Acids / analysis,  chemical synthesis
Aspartic Acid / analysis,  chemical synthesis
Carbon Isotopes
Chromatography, Gas / methods
Evolution, Chemical*
Glycine / analysis,  chemical synthesis
Mass Spectrometry / methods
Valine / analysis,  chemical synthesis
beta-Alanine / analysis,  chemical synthesis
Reg. No./Substance:
0/Amino Acids; 0/Aminoisobutyric Acids; 0/Carbon Isotopes; 107-95-9/beta-Alanine; 56-40-6/Glycine; 56-41-7/Alanine; 56-84-8/Aspartic Acid; 595-40-4/isovaline; 7004-03-7/Valine
M H Engel / U OK, Norman

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Characteristics and formation of amino acids and hydroxy acids of the Murchison meteorite.
Next Document:  Kinetic and mechanistic analysis of nonenzymatic, template-directed oligoribonucleotide ligation.