| Stable isotope analysis at the molecular level: a new approach for determining the origins of amino acids in the Murchison meteorite. | |
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MedLine Citation:
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PMID: 11539266 Owner: NASA Status: MEDLINE |
Abstract/OtherAbstract:
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A combined gas chromatography/isotope ratio mass spectrometry (GC/IRMS) method has been developed that permits the direct stable carbon isotope analysis of N(O)-trifluoroacetyl-isopropyl esters of individual amino acids and their respective enantiomers at nanomole abundances. Calculation of the original delta 13C values of the amino acids is accomplished via a correction for the carbon introduced during the derivatization process. Previous GC/IRMS analyses of individual amino acids in the non-hydrolyzed water extract of an interior sample of a Murchison meteorite stone revealed an enrichment in 13C relative to terrestrial organic matter, in agreement with previous findings for bulk extracts. The range of amino acid delta 13C values (+5 to +30%, PDB) suggests possible kinetic effects during synthesis. In this study, an apparent kinetic isotope effect was also observed for the amino acid products of a spark discharge experiment. These preliminary results are supportive of a similar mechanism for the abiotic synthesis of amino acids in the Murchison meteorite. |
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Authors:
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M H Engel; S A Macko; Y Qian; J A Silfer |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Advances in space research : the official journal of the Committee on Space Research (COSPAR) Volume: 15 ISSN: 0273-1177 ISO Abbreviation: Adv Space Res Publication Date: 1995 Mar |
Date Detail:
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Created Date: 1997-03-21 Completed Date: 1997-03-21 Revised Date: 2007-07-17 |
Medline Journal Info:
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Nlm Unique ID: 9878935 Medline TA: Adv Space Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 99-106 Citation Subset: S |
Affiliation:
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School of Geology and Geophysics, The University of Oklahoma, Norman 73019, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alanine
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analysis,
chemical synthesis Amino Acids / analysis, chemical synthesis* Aminoisobutyric Acids / analysis, chemical synthesis Aspartic Acid / analysis, chemical synthesis Carbon Isotopes Chromatography, Gas / methods Evolution, Chemical* Exobiology Glycine / analysis, chemical synthesis Mass Spectrometry / methods Meteoroids* Valine / analysis, chemical synthesis beta-Alanine / analysis, chemical synthesis |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Aminoisobutyric Acids; 0/Carbon Isotopes; 107-95-9/beta-Alanine; 56-40-6/Glycine; 56-41-7/Alanine; 56-84-8/Aspartic Acid; 595-40-4/isovaline; 7004-03-7/Valine |
| Investigator | |
Investigator/Affiliation:
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M H Engel / U OK, Norman |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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