Document Detail

Stable isotope analysis at the molecular level: a new approach for determining the origins of amino acids in the Murchison meteorite.
MedLine Citation:
PMID:  11539266     Owner:  NASA     Status:  MEDLINE    
A combined gas chromatography/isotope ratio mass spectrometry (GC/IRMS) method has been developed that permits the direct stable carbon isotope analysis of N(O)-trifluoroacetyl-isopropyl esters of individual amino acids and their respective enantiomers at nanomole abundances. Calculation of the original delta 13C values of the amino acids is accomplished via a correction for the carbon introduced during the derivatization process. Previous GC/IRMS analyses of individual amino acids in the non-hydrolyzed water extract of an interior sample of a Murchison meteorite stone revealed an enrichment in 13C relative to terrestrial organic matter, in agreement with previous findings for bulk extracts. The range of amino acid delta 13C values (+5 to +30%, PDB) suggests possible kinetic effects during synthesis. In this study, an apparent kinetic isotope effect was also observed for the amino acid products of a spark discharge experiment. These preliminary results are supportive of a similar mechanism for the abiotic synthesis of amino acids in the Murchison meteorite.
M H Engel; S A Macko; Y Qian; J A Silfer
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Advances in space research : the official journal of the Committee on Space Research (COSPAR)     Volume:  15     ISSN:  0273-1177     ISO Abbreviation:  Adv Space Res     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1997-03-21     Completed Date:  1997-03-21     Revised Date:  2007-07-17    
Medline Journal Info:
Nlm Unique ID:  9878935     Medline TA:  Adv Space Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  99-106     Citation Subset:  S    
School of Geology and Geophysics, The University of Oklahoma, Norman 73019, USA.
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MeSH Terms
Alanine / analysis,  chemical synthesis
Amino Acids / analysis,  chemical synthesis*
Aminoisobutyric Acids / analysis,  chemical synthesis
Aspartic Acid / analysis,  chemical synthesis
Carbon Isotopes
Chromatography, Gas / methods
Evolution, Chemical*
Glycine / analysis,  chemical synthesis
Mass Spectrometry / methods
Valine / analysis,  chemical synthesis
beta-Alanine / analysis,  chemical synthesis
Reg. No./Substance:
0/Amino Acids; 0/Aminoisobutyric Acids; 0/Carbon Isotopes; 107-95-9/beta-Alanine; 56-40-6/Glycine; 56-41-7/Alanine; 56-84-8/Aspartic Acid; 595-40-4/isovaline; 7004-03-7/Valine
M H Engel / U OK, Norman

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