Document Detail


Stable intronic sequence RNA (sisRNA), a new class of noncoding RNA from the oocyte nucleus of Xenopus tropicalis.
MedLine Citation:
PMID:  23154985     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To compare nuclear and cytoplasmic RNA from a single cell type, free of cross-contamination, we studied the oocyte of the frog Xenopus tropicalis, a giant cell with an equally giant nucleus. We isolated RNA from manually dissected nuclei and cytoplasm of mature oocytes and subjected it to deep sequencing. Cytoplasmic mRNA consisted primarily of spliced exons derived from ∼6700 annotated genes. Nearly all of these genes were represented in the nucleus by intronic sequences. However, unspliced nascent transcripts were not detected. Inhibition of transcription or splicing for 1-2 d had little or no effect on the abundance of nuclear intronic sequences, demonstrating that they are unusually stable. RT-PCR analysis showed that these stable intronic sequences are transcribed from the coding strand and that a given intron can be processed into more than one molecule. Stable intronic sequence RNA (sisRNA) from the oocyte nucleus constitutes a new class of noncoding RNA. sisRNA is detectable by RT-PCR in samples of total RNA from embryos up to the mid-blastula stage, when zygotic transcription begins. Storage of sisRNA in the oocyte nucleus and its transmission to the developing embryo suggest that it may play important regulatory roles during oogenesis and/or early embryogenesis.
Authors:
Eugene J Gardner; Zehra F Nizami; C Conover Talbot; Joseph G Gall
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Genes & development     Volume:  26     ISSN:  1549-5477     ISO Abbreviation:  Genes Dev.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-16     Completed Date:  2013-01-17     Revised Date:  2014-05-21    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2550-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cell Nucleus / genetics*
Cytoplasm / genetics,  metabolism
Exons / genetics
Gene Expression Regulation, Developmental
Introns / genetics
Molecular Sequence Data
Oocytes / metabolism*
RNA Splicing / genetics
RNA Stability
RNA, Messenger / genetics
RNA, Untranslated / genetics*,  metabolism
Xenopus / embryology*,  genetics*,  metabolism
Grant Support
ID/Acronym/Agency:
P40 OD010997/OD/NIH HHS; R01 GM33397/GM/NIGMS NIH HHS; T32 GM007231/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/RNA, Untranslated
Comments/Corrections

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