| Stable intronic sequence RNA (sisRNA), a new class of noncoding RNA from the oocyte nucleus of Xenopus tropicalis. | |
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MedLine Citation:
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PMID: 23154985 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To compare nuclear and cytoplasmic RNA from a single cell type, free of cross-contamination, we studied the oocyte of the frog Xenopus tropicalis, a giant cell with an equally giant nucleus. We isolated RNA from manually dissected nuclei and cytoplasm of mature oocytes and subjected it to deep sequencing. Cytoplasmic mRNA consisted primarily of spliced exons derived from ∼6700 annotated genes. Nearly all of these genes were represented in the nucleus by intronic sequences. However, unspliced nascent transcripts were not detected. Inhibition of transcription or splicing for 1-2 d had little or no effect on the abundance of nuclear intronic sequences, demonstrating that they are unusually stable. RT-PCR analysis showed that these stable intronic sequences are transcribed from the coding strand and that a given intron can be processed into more than one molecule. Stable intronic sequence RNA (sisRNA) from the oocyte nucleus constitutes a new class of noncoding RNA. sisRNA is detectable by RT-PCR in samples of total RNA from embryos up to the mid-blastula stage, when zygotic transcription begins. Storage of sisRNA in the oocyte nucleus and its transmission to the developing embryo suggest that it may play important regulatory roles during oogenesis and/or early embryogenesis. |
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Authors:
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Eugene J Gardner; Zehra F Nizami; C Conover Talbot; Joseph G Gall |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Genes & development Volume: 26 ISSN: 1549-5477 ISO Abbreviation: Genes Dev. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-16 Completed Date: 2013-01-17 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 8711660 Medline TA: Genes Dev Country: United States |
Other Details:
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Languages: eng Pagination: 2550-9 Citation Subset: IM |
Affiliation:
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Department of Embryology, Carnegie Institution for Science, Baltimore, Maryland 21218, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cell Nucleus / genetics* Cytoplasm / genetics, metabolism Exons / genetics Gene Expression Regulation, Developmental Introns / genetics Molecular Sequence Data Oocytes / metabolism* RNA Splicing / genetics RNA Stability RNA, Messenger / genetics RNA, Untranslated / genetics*, metabolism Xenopus / embryology*, genetics*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM33397/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/RNA, Untranslated |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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