Document Detail

A stable cranial neural crest cell line from mouse.
MedLine Citation:
PMID:  22889333     Owner:  NLM     Status:  MEDLINE    
Cranial neural crest cells give rise to ectomesenchymal derivatives such as cranial bones, cartilage, smooth muscle, dentin, as well as melanocytes, corneal endothelial cells, and neurons and glial cells of the peripheral nervous system. Previous studies have suggested that although multipotent stem-like cells may exist during the course of cranial neural crest development, they are transient, undergoing lineage restriction early in embryonic development. We have developed culture conditions that allow cranial neural crest cells to be grown as multipotent stem-like cells. With these methods, we obtained 2 independent cell lines, O9-1 and i10-1, which were derived from mass cultures of Wnt1-Cre; R26R-GFP-expressing cells. These cell lines can be propagated and passaged indefinitely, and can differentiate into osteoblasts, chondrocytes, smooth muscle cells, and glial cells. Whole-genome expression profiling of O9-1 cells revealed that this line stably expresses stem cell markers (CD44, Sca-1, and Bmi1) and neural crest markers (AP-2α, Twist1, Sox9, Myc, Ets1, Dlx1, Dlx2, Crabp1, Epha2, and Itgb1). O9-1 cells are capable of contributing to cranial mesenchymal (osteoblast and smooth muscle) neural crest fates when injected into E13.5 mouse cranial tissue explants and chicken embryos. These results suggest that O9-1 cells represent multipotent mesenchymal cranial neural crest cells. The O9-1 cell line should serve as a useful tool for investigating the molecular properties of differentiating cranial neural crest cells.
Mamoru Ishii; Athena C Arias; Liqiong Liu; Yi-Bu Chen; Marianne E Bronner; Robert E Maxson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-04
Journal Detail:
Title:  Stem cells and development     Volume:  21     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-08     Completed Date:  2013-04-19     Revised Date:  2013-12-05    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3069-80     Citation Subset:  IM    
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MeSH Terms
Antigens, CD44 / metabolism
Biological Markers / metabolism
Cell Culture Techniques / methods*
Cell Differentiation*
Cell Line*
Cell Movement
Chick Embryo
Culture Media / metabolism
Flow Cytometry
Gene Expression Profiling
Myocytes, Smooth Muscle / cytology,  metabolism
Neural Crest / cytology*
Neuroglia / cytology,  metabolism
Skull / cytology*,  metabolism
Grant Support
R01 DE016320/DE/NIDCR NIH HHS; R01 DE019650/DE/NIDCR NIH HHS; R01DE016320/DE/NIDCR NIH HHS; R01DE019650/DE/NIDCR NIH HHS; TB1-01192//Canadian Institutes of Health Research
Reg. No./Substance:
0/Antigens, CD44; 0/Biological Markers; 0/Cd44 protein, mouse; 0/Culture Media

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