| A Stable Cranial Neural Crest Cell Line from Mouse. | |
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MedLine Citation:
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PMID: 22889333 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Cranial neural crest cells give rise to ectomesenchymal derivatives such as cranial bones, cartilage, smooth muscle, dentin, as well as melanocytes, corneal endothelial cells, and neurons and glial cells of the peripheral nervous system. Previous studies have suggested that although multipotent stem-like cells may exist during the course of cranial neural crest development, they are transient, undergoing lineage restriction early in embryonic development. We have developed culture conditions that allow cranial neural crest cells to be grown as multipotent stem-like cells. With these methods, we obtained two independent cell lines, O9-1 and i10-1, which were derived from mass cultures of Wnt1-Cre; R26R-GFP expressing cells. These cell lines can be propagated and passaged indefinitely, and can differentiate into osteoblasts, chondrocytes, smooth muscle cells and glial cells. Whole genome expression profiling of O9-1 revealed that this line stably expresses stem cell markers (CD44, Sca-1, and Bmi1), and neural crest markers (AP-2α, Twist1, Sox9, Myc, Ets1, Dlx1, Dlx2, Crabp1, Epha2, and Itgb1). O9-1 cells are capable of contributing to cranial mesenchymal (osteoblast and smooth muscle) neural crest fates when injected into E13.5 mouse cranial tissue explants and chicken embryos. These results suggest that O9-1 cells represent multipotent mesenchymal cranial neural crest cells. The O9-1 cell line should serve as a useful tool for investigating the molecular properties of differentiating cranial neural crest cells. |
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Authors:
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Mamoru Ishii; Athena C Arias; Liqiong Liu; Yi-Bu Chen; Marianne Bronner; Robert E Maxson |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-13 |
Journal Detail:
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Title: Stem cells and development Volume: - ISSN: 1557-8534 ISO Abbreviation: Stem Cells Dev. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101197107 Medline TA: Stem Cells Dev Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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USC/Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Biochemistry and Molecular Biology, LA, California, United States; ishii@usc.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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