| Stabilization and electrophoretic analysis of meiotic recombination intermediates in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 19799185 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Joint Molecule (JM) recombination intermediates result from DNA strand-exchange between homologous chromosomes. Physical monitoring of JM formation in budding yeast has provided a wealth of information about the timing and mechanism of meiotic recombination. These assays are especially informative when applied to the analysis of mutants for which genetic analysis of recombination is impossible, i.e. mutants that die during meiosis. This chapter describes three distinct methods to stabilize JMs against thermally driven dissolution as well as electrophoretic approaches to resolve and detect JMs at two well-characterized recombination hotspots. |
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Authors:
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Steve D Oh; Lea Jessop; Jessica P Lao; Thorsten Allers; Michael Lichten; Neil Hunter |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Review |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 557 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-10-05 Completed Date: 2009-11-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 209-34 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University of California, Davis, CA, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Chromosomal Instability / physiology* DNA, Fungal / isolation & purification Electrophoresis, Agar Gel / methods Meiosis / genetics* Models, Biological Recombination, Genetic / genetics* Restriction Mapping / methods Saccharomyces cerevisiae / chemistry, genetics* |
| Grant Support | |
ID/Acronym/Agency:
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GM074223/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Fungal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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