| Stabilization of amorphous calcium carbonate by phosphate rich organic matrix proteins and by single phosphoamino acids. | |
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MedLine Citation:
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PMID: 20416381 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stable amorphous calcium carbonate (ACC) is a unique material produced naturally exclusively as a biomineral. It was demonstrated that proteins extracted from biogenic stable ACC induce and stabilize synthetic ACC in vitro. Polyphosphate molecules were similarly shown to induce amorphous calcium carbonate formation in vitro. Accordingly, we tested the hypothesis that biogenic ACC induction and stabilization is mediated by the phosphorylated residues of phosphoproteins. We show that extracellular organic matrix extracted from gastroliths of the red claw crayfish Cherax quadricarinatus induce stable ACC formation in vitro. The proteinaceous fraction of this organic matrix is highly phosphorylated and is incorporated into the ACC mineral phase during precipitation. We have identified the major phosphoproteins of the organic matrix and showed that they have high calcium binding capacity. Based on the above, in vitro precipitation experiments with single phosphoamino acids were performed, indicating that phosphoserine or phosphothreonine alone can induce the formation of highly stable ACC. The results indicate that phosphoproteins may play a major role in the control of ACC formation and stabilization and that their phosphoamino acid moieties are key components in this process. |
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Authors:
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Shmuel Bentov; Simy Weil; Lilah Glazer; Amir Sagi; Amir Berman |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-21 |
Journal Detail:
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Title: Journal of structural biology Volume: 171 ISSN: 1095-8657 ISO Abbreviation: J. Struct. Biol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-06-14 Completed Date: 2010-09-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9011206 Medline TA: J Struct Biol Country: United States |
Other Details:
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Languages: eng Pagination: 207-15 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Biotechnology Engineering, Ben-Gurion University of the Negev, 84105, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Astacoidea / chemistry, metabolism Calcium Carbonate / chemistry, metabolism* Male Microscopy, Electron, Scanning Microscopy, Electron, Transmission Phosphoamino Acids / chemistry, metabolism* Phosphoproteins / chemistry*, metabolism* Spectrum Analysis, Raman |
| Chemical | |
Reg. No./Substance:
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0/Phosphoamino Acids; 0/Phosphoproteins; 471-34-1/Calcium Carbonate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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