Document Detail

Stabilization of D-amino-acid oxidase from Trigonopsis variabilis by manganese dioxide.
MedLine Citation:
PMID:  10983233     Owner:  NLM     Status:  MEDLINE    
Stabilization of immobilized D-amino-acid oxidase was achieved as follows. Yeast Trigonopsis variabilis producing D-amino-acid oxidase was used to deaminate cephalosporin C to glutaryl-7-aminocephalosporanic acid. Permeabilized cells were co-immobilized with manganese dioxide by entrapment in (poly)acrylamide gel so that hydrogen peroxide, liberated in the reaction, could be partially deactivated and both the enzyme and the substrate could be stabilized. Activity of entrapped cells was determined by HPLC and enzyme flow microcalorimetry. The process was evaluated in terms of activity, immobilization yield, storage stability and oxo-product formation by immobilized preparations. The storage stability of immobilized biocatalysts with MnO2 was nearly doubled and production of 2-oxoadipyl-7-aminocephalosporanic acid was 2-3-fold higher than by entrapped cells without MnO2. Glutaryl-7-aminocephalosporanic acid can be easily obtained from the resulting oxo-product by a non-enzymic reaction via externally added hydrogen peroxide.
A Vikartovská-Welwardová; E Michalková; P Gemeiner; L Welward
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Folia microbiologica     Volume:  44     ISSN:  0015-5632     ISO Abbreviation:  Folia Microbiol. (Praha)     Publication Date:  1999  
Date Detail:
Created Date:  2000-10-23     Completed Date:  2000-10-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376757     Medline TA:  Folia Microbiol (Praha)     Country:  CZECH REPUBLIC    
Other Details:
Languages:  eng     Pagination:  380-4     Citation Subset:  IM    
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.
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MeSH Terms
Ascomycota / enzymology*
Cephalosporins / metabolism
Chromatography, High Pressure Liquid
D-Amino-Acid Oxidase / metabolism*
Enzyme Stability / drug effects
Enzymes, Immobilized
Manganese Compounds / pharmacology
Oxides / pharmacology
Reg. No./Substance:
0/Cephalosporins; 0/Enzymes, Immobilized; 0/Manganese Compounds; 0/Oxides; 1317-35-7/manganese oxide; 61-24-5/cephalosporin C; EC Oxidase

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