| Stability of water-soluble carbodiimides in aqueous solution. | |
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MedLine Citation:
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PMID: 2158246 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A dimethylbarbituric acid reagent has been used to follow the kinetics of loss of two water-soluble carbodiimides, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and the structurally related 1-ethyl-3-(4-azonia-4,4-dimethylpentyl) carbodiimide (EAC), in aqueous solution as a function of pH and added chemical reagents. In 50 mM 2-(N-morpholino)ethanesulfonic acid at 25 degrees C, EDC has t1/2 values of 37, 20, and 3.9 h at pH 7.0, 6.0, and 5.0, respectively, while the corresponding values for EAC are 12, 2.9, and 0.32 h. Iodide, bromide, or chloride, at 0.1 M, has very little or no effect on carbodiimide stability. However, 0.1 M glycine methyl ester or 0.1 M ethylenediamine causes a significant increase in the rate of loss of EAC and EDC, while the presence of 0.1 M phosphate, 0.1 M hydroxylamine, or 0.01 M ATP decreases the half-lives to less than or equal to 0.4 h at all pH values. |
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Authors:
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M A Gilles; A Q Hudson; C L Borders |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Analytical biochemistry Volume: 184 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1990 Feb |
Date Detail:
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Created Date: 1990-05-16 Completed Date: 1990-05-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 244-8 Citation Subset: IM |
Affiliation:
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Department of Chemistry, College of Wooster, Ohio 44691. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkanesulfonates Alkanesulfonic Acids* Carbodiimides* Ethyldimethylaminopropyl Carbodiimide Hydrogen-Ion Concentration Hydrolysis Morpholines Solutions Water |
| Chemical | |
Reg. No./Substance:
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0/Alkanesulfonates; 0/Alkanesulfonic Acids; 0/Carbodiimides; 0/Morpholines; 0/Solutions; 1892-57-5/Ethyldimethylaminopropyl Carbodiimide; 4432-31-9/2-(N-morpholino)ethanesulfonic acid; 45024-77-9/1-ethyl-3-(4-azonia-4,4-dimethylpentyl)carbodiimide; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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