Document Detail


Stability and reliability of plasma level of lipid biomarkers and their correlation with dietary fat intake.
MedLine Citation:
PMID:  18219092     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The reliability and stability of plasma lipid biomarkers and their association with dietary fat intake were evaluated among 48 subjects who were randomly chosen from the participants of a validation study of the population-based cohort, the Shanghai Men's Health Study (SMHS). Four spot blood samples, one taken each season, were measured for total cholesterol, triglyceride, HDL-cholesterol, and LDL-cholesterol levels. The reliability and stability of these measurements were assessed by intraclass correlation coefficients (ICC) and by the correlations between a randomly chosen measurement with the mean of measurements across seasons using a bootstrap approach. The median levels for total cholesterol, triglycerides, HDL-cholesterol, and LDL-cholesterol were 177.5, 164.5, 41.0, and 102.5 (mg/dl), respectively. The ICCs of the biomarkers ranged from 0.58 (LDL-cholesterol) to 0.83 (HDL-cholesterol). The correlation between randomly chosen spot measurements and the mean measurement were 0.91, 0.86, 0.93, and 0.83 for total cholesterol, triglycerides, HDL-cholesterol, and LDL-cholesterol, respectively. The correlations of lipid biomarkers with dietary fat intake and other lifestyle factors were comparable to other previous reports. In conclusion, this study suggests that measurements of lipid biomarkers from a single spot blood sample are a good representation of the average blood levels of these biomarkers in the study population and could be a useful tool for epidemiological studies.
Authors:
Sang-Ah Lee; Wanqing Wen; Yong-Bing Xiang; Sergio Fazio; Macrae F Linton; Qiuyin Cai; Dake Liu; Wei Zheng; Xiao-Ou Shu
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Disease markers     Volume:  24     ISSN:  0278-0240     ISO Abbreviation:  Dis. Markers     Publication Date:  2008  
Date Detail:
Created Date:  2008-01-25     Completed Date:  2008-04-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8604127     Medline TA:  Dis Markers     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  73-9     Citation Subset:  IM    
Affiliation:
Department of Medicine, Vanderbilt Epidemiology Center, Vanderbilt University Medical Center and Vanderbilt Ingram Cancer Center, Nashville, TN 37203-1738, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Biological Markers
Cholesterol, HDL / blood
Cholesterol, LDL / blood
Dietary Fats / administration & dosage*
Humans
Lipids / blood*
Male
Middle Aged
Reproducibility of Results
Triglycerides / blood
Grant Support
ID/Acronym/Agency:
R01CA82729/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Cholesterol, HDL; 0/Cholesterol, LDL; 0/Dietary Fats; 0/Lipids; 0/Triglycerides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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