Document Detail

Stability of the prion protein-encoding (PRNP) gene in HeLa cells.
MedLine Citation:
PMID:  12623063     Owner:  NLM     Status:  MEDLINE    
To assess the risk of the de novo emergence of the agent of transmissible spongiform encephalopathies in cultured cells, we examined the stability of the prion protein-encoding (PRNP) gene in HeLa cells and in cultures contaminated with HeLa cells that have been passaged extensively for over 50 years. Various sub-lineages of HeLa cells showed that some contained a mixture of a truncated PRNP gene (R3-R4 deletion) and a full-length PRNP gene, while others were homozygous for the R3-R4 deletion. That finding suggests that the progenitor of several popular sub-lineages of HeLa must have lost part or all of chromosome 20 early in the history of HeLa cells. No mutations were found in the PRNP genes. We conclude that the spontaneous appearance of mutations leading to expression of abnormal prion proteins in continuously passaged heteroploid cell lines is unlikely to pose a substantial risk for the safe production of biologicals in such cells.
Georgios Amexis; Jeanette Ridge; Larisa Cervenakova; Joan C Enterline; Konstantin M Chumakov; David M Asher
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biologicals : journal of the International Association of Biological Standardization     Volume:  31     ISSN:  1045-1056     ISO Abbreviation:  Biologicals     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-07     Completed Date:  2003-11-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9004494     Medline TA:  Biologicals     Country:  England    
Other Details:
Languages:  eng     Pagination:  83-6     Citation Subset:  IM    
Center for Biologics Evaluation and Research, United States Food and Drug Administration, 1401 Rockville Pike, HFM-313, Rockville, MD 20852-1448, USA.
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MeSH Terms
Base Sequence
DNA Primers
Hela Cells
Polymerase Chain Reaction
Prions / genetics*
Reg. No./Substance:
0/DNA Primers; 0/Prions

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