| Stability of immunophenotypic markers in fixed peripheral blood for extended analysis using flow cytometry. | |
| | |
MedLine Citation:
|
PMID: 20875824 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The analysis of whole blood samples by flow cytometry for pharmacodynamic and biomarker assessments in clinical studies has been limited by the necessity to test these samples within a short time frame after blood collection. In most clinical studies, blood specimens are shipped to a centralized testing facility; it is critical to demonstrate specimen stability over a period of time which will encompass the time elapsed between specimen collection and testing. A possible solution to overcome this limitation is the use of a fixative to preserve the cell surface antigen stability in whole blood. We examined the stability of markers for T lymphocytes (CD3, CD4, CD8, CD45RA, and CD45RO), B lymphocytes (CD19), NK cells (CD16+CD56), activation (CD25 and HLA-DR), chemokine receptors (CCR5 and CXCR3) and skin homing (CLA) in fixed blood over 7 days and used this information to select the markers for global clinical studies. These assays with selected markers were further validated using fit-for-purpose approach (Lee et al., 2006) and to set the sample acceptability criteria for use in clinical sample testing. Most of the markers examined were stable when collected in Cyto-Chex® BCT for one week with the exception of the activation markers on T cells. |
| | |
Authors:
|
Cuc Davis; Xiaoying Wu; Weimin Li; Hongtao Fan; Manjula Reddy |
Related Documents
:
|
3582064 - On multiparameter data analysis in flow cytometry. 15688354 - Phytoplankton monitoring by high performance flow cytometry: a successful approach? 18206724 - Flow cytometric assessment of homeostatic aging of reticulocytes in rats. 10685514 - Flow cytometry as a tool to monitor the disturbance of phagocytosis in the clam mya are... 15364594 - Transition of cord blood lead level, 1985-2002, in the taipei area and its determinants... 11910524 - Adrenergic supersensitivity of rabbit choroidal blood vessels after sympathetic denerva... |
Publication Detail:
|
Type: Journal Article; Validation Studies Date: 2010-09-27 |
Journal Detail:
|
Title: Journal of immunological methods Volume: 363 ISSN: 1872-7905 ISO Abbreviation: J. Immunol. Methods Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2010-12-17 Completed Date: 2011-01-24 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 158-65 Citation Subset: IM |
Copyright Information:
|
Published by Elsevier B.V. |
Affiliation:
|
Oncology Biomarkers, Centocor R&D, 145 King of Prussia Road, Radnor, PA 19087, USA. cdavis21@its.jnj.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
B-Lymphocytes
/
immunology* Flow Cytometry / methods* Humans Immunophenotyping / methods* Killer Cells, Natural / immunology* Leukocytes, Mononuclear / immunology Specimen Handling / methods* Statistics, Nonparametric T-Lymphocytes / immunology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecular cloning and characterization of preproorexin in winter skate (Leucoraja ocellata).
Next Document: Migration and proliferation of human mesenchymal stem cells is stimulated by different regions of th...