| Stability of gels formed following coagulation of Limulus amebocyte lysate: lack of covalent crosslinking of coagulin. | |
| | |
MedLine Citation:
|
PMID: 2781519 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Incubation of lysates prepared from amebocytes of the horseshoe crab (Limulus polyphemus) with bacterial endotoxin results in coagulation and formation of a solid gel. Although Limulus gels remained solid indefinitely, if undisturbed, they were easily disrupted by mechanical agitation. Chemical solubility studies of gelled lysates demonstrated rapid solubilization of gels in monochloroacetic acid, a property of clots that have not been covalently stabilized; but in contrast demonstrated resistance to solubilization by urea, a property of stabilized clots. Analysis of solubilized proteins by polyacrylamide gel electrophoresis in SDS demonstrated coagulin, the designation for the activated form of coagulogen (the clottable protein) that forms a gel, only in samples derived from clotted lysate that had been previously incubated with monochloroacetic acid, but not in samples following incubation with urea, confirming the results of the chemical solubility studies. Enzymatic assays for transpeptidase (Factor XIII-like) activity in either native or gelled Limulus lysates were negative. Furthermore, analysis for covalently crosslinked peptides in gelled coagulin confirmed the absence of intermolecular gamma-glutamyl-epsilon-lysyl bonds. Therefore, the stable gels formed following coagulation of Limulus lysate by bacterial endotoxin are not covalently crosslinked. |
| | |
Authors:
|
R I Roth; J C Chen; J Levin |
Related Documents
:
|
3897859 - Glycosidases of trichinella spiralis. 50389 - Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins of newbonr rat sk... 1536659 - Characterization and cellular distribution of acidic peptide and oligosaccharide metal-... 923519 - Subunit structure of 27 s thyroid iodoprotein. 15255339 - Isolation and properties of glycosylated pig prolactin (g-prl). 13818289 - Subfractionation of human serum enzymes. 3897859 - Glycosidases of trichinella spiralis. 2897459 - Compatibility of intravenous fat emulsions with prodrug amino acids. 1567229 - Oligouronide signaling of proteinase inhibitor genes in plants: structure-activity rela... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Thrombosis research Volume: 55 ISSN: 0049-3848 ISO Abbreviation: Thromb. Res. Publication Date: 1989 Jul |
Date Detail:
|
Created Date: 1989-10-20 Completed Date: 1989-10-20 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 0326377 Medline TA: Thromb Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 25-36 Citation Subset: IM |
Affiliation:
|
Department of Laboratory Medicine, University of California School of Medicine, San Francisco. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Coagulation Tests Chemical Phenomena Chemistry Endotoxins / pharmacology Gels Horseshoe Crabs / physiology* Nephelometry and Turbidimetry Peptidyl Transferases / metabolism Solubility Thromboplastin* |
| Chemical | |
Reg. No./Substance:
|
0/Endotoxins; 0/Gels; 67924-63-4/endotoxin, Escherichia coli; 9035-58-9/Thromboplastin; EC 2.3.2.12/Peptidyl Transferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Rapid purification of factor IX, factor X and prothrombin by immunoaffinity and ion exchange chromat...
Next Document: Identification of the 1.4 kb and 4.0 kb messages for the lipoprotein associated coagulation inhibito...