Stability of early-stage amyloid-β(1-42) aggregation species.

Stability of early-stage amyloid-β(1-42) aggregation species.

MedLine Citation:	PMID: 22944394 Owner: NLM Status: MEDLINE
Abstract/OtherAbstract:	Accumulation of aggregated amyloid-β protein (Aβ) is an important feature of Alzheimer's disease. There is significant interest in understanding the initial steps of Aβ aggregation due to the recent focus on soluble Aβ oligomers. In vitro studies of Aβ aggregation have been aided by the use of conformation-specific antibodies which recognize shape rather than sequence. One of these, OC antiserum, recognizes certain elements of fibrillar Aβ across a broad range of sizes. We have observed the presence of these fibrillar elements at very early stages of Aβ incubation. Using a dot blot assay, OC-reactivity was found in size exclusion chromatography (SEC)-purified Aβ(1-42) monomer fractions immediately after isolation (early-stage). The OC-reactivity was not initially observed in the same fractions for Aβ(1-40) or the aggregation-restricted Aβ(1-42) L34P but was detected within 1-2weeks of incubation. Stability studies demonstrated that early-stage OC-positive Aβ(1-42) aggregates were resistant to 4M urea or guanidine hydrochloride but sensitive to 1% sodium dodecyl sulfate (SDS). Interestingly, the sensitivity to SDS diminished over time upon incubation of the SEC-purified Aβ(1-42) solution at 4°C. Within 6-8days the OC-positive Aβ42 aggregates were resistant to SDS denaturation. The progression to, and development of, SDS resistance for Aβ(1-42) occurred prior to thioflavin T fluorescence. In contrast, Aβ(1-40) aggregates formed after 6days of incubation were sensitive to both urea and SDS. These findings reveal information on some of the earliest events in Aβ aggregation and suggest that it may be possible to target early-stage aggregates before they develop significant stability.

Authors:	Kelley A Coalier; Geeta S Paranjape; Sanjib Karki; Michael R Nichols
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Publication Detail:	Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-08-25
Journal Detail:	Title: Biochimica et biophysica acta Volume: 1834 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2013 Jan
Date Detail:	Created Date: 2012-12-26 Completed Date: 2013-03-18 Revised Date: 2013-04-16
Medline Journal Info:	Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands
Other Details:	Languages: eng Pagination: 65-70 Citation Subset: IM
Copyright Information:	Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:	Department of Chemistry and Biochemistry and Center for Nanoscience, University of Missouri-St. Louis, MO 63121, USA.
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MeSH Terms
Descriptor/Qualifier:	Amyloid / chemistry* Amyloid beta-Peptides / chemistry* Antibodies / chemistry Guanidine / chemistry Humans Peptide Fragments / chemistry* Protein Stability Sodium Dodecyl Sulfate / chemistry Urea / chemistry
Grant Support
ID/Acronym/Agency:	R15AG033913/AG/NIA NIH HHS
Chemical
Reg. No./Substance:	0/Amyloid; 0/Amyloid beta-Peptides; 0/Antibodies; 0/Peptide Fragments; 0/amyloid beta-protein (1-42); 113-00-8/Guanidine; 151-21-3/Sodium Dodecyl Sulfate; 57-13-6/Urea

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