| Stability of diazepam in blood samples at different storage conditions and in the presence of alcohol. | |
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MedLine Citation:
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PMID: 21549532 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Diazepam is one of the mostly used benzodiazepines and it is frequently analyzed in different biological samples, especially blood samples. The diazepam stability in the sample matrices is an important factor regarding reliable data obtaining. The storage is the main factor determining the stability of diazepam in blood samples and it is the object of the study presented. Remaining diazepam amount in spiked whole blood and plasma samples were tested at different storage temperatures, in the absence or presence of sodium fluoride as stabilizer as well as the influence of ethanol on diazepam stability was evaluated. The results of the study indicated that the temperature is the main storage factor affecting diazepam stability. In the fluoride stabilized blood samples the amount of diazepam decreases up to 85% of initial level when stored at -20°C for the period of testing (12 weeks). The presence of low (0.5g/L) or high (3g/L) ethanol concentrations influences the stability of diazepam at -20°C. In whole blood samples, the combination of sodium fluoride and ethanol decreases additionally (15-25%) the concentration of the analyte. Freeze-thaw experiments of whole blood samples show about 5-9% decrease in diazepam concentration after the first cycle. The freeze-thaw experiments on plasma samples, containing ethanol and/or fluoride show insignificant decreases of analyte concentration. Further experiments on benzodiazepines stability at different storage conditions or in combination of different factors should be undertaken in forensic toxicology to ensure the data quality, their reliability and reproducibility. |
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Authors:
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V N Atanasov; S Stoykova; A Runiov; T Dimitrova; D Aleksandrova; S Tsakovski; M Mitewa |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-4 |
Journal Detail:
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Title: Forensic science international Volume: - ISSN: 1872-6283 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7902034 Medline TA: Forensic Sci Int Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Analytical, Faculty of Chemistry, Sofia University "St. Kliment Ohridsky", 1 "J. Bourchier" Blvd., 1164 Sofia, Bulgaria. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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