| Stability of cyclopropane and conjugated linoleic acids during fatty acid quantification in lactic acid bacteria. | |
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MedLine Citation:
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PMID: 10580338 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Seven methods commonly used for fatty acid analysis of microorganisms and foods were compared to establish the best for the analysis of lyophilized lactic acid bacteria. One of these methods involves fat extraction followed by methylation of fatty acids, while the other methods use a direct methylation of the samples, under different operating conditions (e.g., reaction temperature and time, reagents, and pH). Fatty acid methyl esters were identified by gas chromatography-mass spectrometry and quantified by on-column capillary gas chromatography. Two reliable methods for the analysis of fatty acids in bacteria were selected and further improved. They guarantee high recovery of classes of fragile fatty acids, such as cyclopropane and conjugated acids, and a high degree of methylation for all types of fatty acid esters. These two direct methylation methods have already been successfully applied to the analysis of fatty acids in foods. They represent a rapid and highly reliable alternative to classical time- and solvent-consuming methods and they give the fatty acid profile and the amount of each fatty acid. Using these methods, conjugated linoleic acids were identified and quantified in lactic acid bacteria. |
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Authors:
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F Dionisi; P A Golay; M Elli; L B Fay |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Lipids Volume: 34 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 1999 Oct |
Date Detail:
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Created Date: 1999-12-27 Completed Date: 1999-12-27 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1107-15 Citation Subset: IM |
Affiliation:
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Nestlé Research Centre, Nestec Ltd., Lausanne, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteria
/
chemistry,
metabolism* Chromatography / methods Cyclopropanes / chemistry Fatty Acids / chemistry*, metabolism* Lactic Acid / metabolism* Linoleic Acid / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Cyclopropanes; 0/Fatty Acids; 2197-37-7/Linoleic Acid; 50-21-5/Lactic Acid; 75-19-4/cyclopropane |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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