Document Detail

Stability of citrate-capped silver nanoparticles in exposure media and their effects on the development of embryonic zebrafish (Danio rerio).
MedLine Citation:
PMID:  23325492     Owner:  NLM     Status:  MEDLINE    
The stability of citrate-capped silver nanoparticles (AgNPs) and the embryonic developmental toxicity were evaluated in the fish test water. Serious aggregation of AgNPs was observed in undiluted fish water (DM-100) in which high concentration of ionic salts exist. However, AgNPs were found to be stable for 7 days in DM-10, prepared by diluting the original fish water (DM-100) with deionized water to 10 %. The normal physiology of zebrafish embryos were evaluated in DM-10 to see if DM-10 can be used as a control vehicle for the embryonic fish toxicity test. As results, DM-10 without AgNPs did not induce any significant adverse effects on embryonic development of zebrafish determined by mortality, hatching, malformations and heart rate. When embryonic toxicity of AgNPs was tested in both DM-10 and in DM-100, AgNPs showed higher toxicity in DM-10 than in DM-100. This means that the big-sized aggregates of AgNPs were low toxic compared to the nano-sized AgNPs. AgNPs induced delayed hatching, decreased heart rate, pericardial edema, and embryo death. Accumulation of AgNPs in the embryo bodies was also observed. Based on this study, citrate-capped AgNPs are not aggregated in DM-10 and it can be used as a control vehicle in the toxicity test of fish embryonic development.
Kwangsik Park; George Tuttle; Federico Sinche; Stacey L Harper
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Archives of pharmacal research     Volume:  36     ISSN:  0253-6269     ISO Abbreviation:  Arch. Pharm. Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-02-11     Completed Date:  2013-07-19     Revised Date:  2014-05-23    
Medline Journal Info:
Nlm Unique ID:  8000036     Medline TA:  Arch Pharm Res     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  125-33     Citation Subset:  IM    
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MeSH Terms
Citric Acid / chemistry
Culture Media / chemistry
Dose-Response Relationship, Drug
Drug Stability
Embryo, Nonmammalian / drug effects*
Embryonic Development / drug effects*
Environmental Exposure
Metal Nanoparticles / chemistry,  toxicity*
Particle Size
Silver / chemistry,  toxicity*
Water Pollutants, Chemical / chemistry,  toxicity*
Zebrafish / embryology*
Grant Support
Reg. No./Substance:
0/Culture Media; 0/Water Pollutants, Chemical; 2968PHW8QP/Citric Acid; 3M4G523W1G/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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